By using a luciferase
reporter assay followed by a heat map representation of the data, we made several findings.
To verify the target, luciferase
reporter was performed in both KLE and AN3CA cells.
We next determined if curcumin inhibits MUC5AC transcription by determining the activity of MUC5AC promoter-driven luciferase
Transient Transfection and Luciferase
Reporter Gene Assay.
To conclusively determine whether [beta]-catenin is a target of miR-200a, we performed a luciferase
reporter assay and constructed luciferase
reporter plasmids carrying the WT or MUT form of the [beta]-catenin 3'-UTR.
Despite its difficulties with absolute quantitation, luciferase
has been used to monitor ATP changes in a variety of cells, such as HEK-293 cells, cardiomyocytes, and neurons (Brovko, 2010).
Blumberg, University of California, Irvine], 80 ng UASx4 TK-luc [contains four copies of the GAL4 upstream activating sequence and the herpes virus thymidine kinase promoter (-105/+51) driving firefly luciferase] and 4 ng of pRL vector (encoding Renilla luciferase
to control for transfection efficiency).
We then showed that Ngn3 could bind to the promoter of Sohlh1andincrease the transcription of Sohlh1 by ChIP and luciferase
reporter gene assay.
For simultaneous determination of promoter activities of ADAM10 and BACE-1 a dual luciferase
reporter assay was employed as previously described (Reinhardt et al.
Anti-GP neutralizing antibodies can inhibit the entry of this pseudoviral particle into cells, thereby inhibiting the expression of luciferase
in the assay.
F2koPB1/WSN) as well as a plasmid that expressed the pseudo-virus RNA (NP/WSN combined with luciferase
) by cellular RNA polymerase I (referred to as PoI-NP-Luc)  and a control reporter plasmid (referred to as pRL-TK).
In addition, the cells utilise the luciferase
reporter gene, which provides a highly sensitive measure of changes in ERR?