A few of these substances include luminol
with concentration of 25 mM and 12 IU/ml HRP were added and the Relative Light Unit (RLU) was assessed for 30 min with 2-min intervals at 37[degrees]C using a microplate reader with luminescence detection technology (Synergy TM HT, BioTek[R] instruments, Inc.
After incubation, 25 ul of serum opsonized zymosan (SOZ) [Fluka, Buchs, Switzerland] and 25 ul of intracellular reactive oxygen species detecting probe, luminol
[Research Organics, Cleveland, OH, USA] were added into each well, except blank wells (containing only HBSS++).
After removing the unbound conjugate by washing, a reagent containing luminogenic substrates (Luminol
derivative and peracid salt) and an electron transfer agent is added to the wells.
Selective detection of dopamine in the presence of uric acid using a gold nanoparticles-poly(luminol
) hybrid film and multi-walled carbon nanotubes with incorporated--cyclodextrin modified glassy carbon electrode.
After addition of western blotting Luminol
reagents (Santa Cruz Biotechnology, USA), signals of proteins were recorded using Bio-Rad ChemiDoc [TM] XRS system (Bio-Rad Laboratories).
During I/R, with the myeloperoxidase-mediated neutrophil activation, the assay of excess production of free radicals was represented by using luminol
chemiluminescence (CL) and lucigenin CL as indicators.
Proteins were visualized using chemiluminescence luminol
reagents (Thermo Scientific Super Signal West Femto Maximum Sensitivity Substrate).
I played performances such as Steven Wilson - Luminol
(live) from the Get All You Deserve Blu-Ray and Watchmaker (live in Frankfurt).
Finally, their antioxidant activities were evaluated by different luminol
CL systems and DPPH radical assay.
Finally, the blots were developed with luminol
reagent (Santa Cruz, CA, USA).
Neutrophil oxidants react with luminol
. The intensity of chemiluminescence is proportional to the endotoxin level in a patient sample [14, 15].