We randomly collected 60 samples from the routine hemostasis laboratory, which included 20 normal, 10 partially lysed
and icteric, and 30 abnormal (with or without anticoagulant) samples [Table 1].
We assessed the sensitivity of this probe by measuring the response of the probe to increasing cAMP concentrations using a lysed
Anterior vitreous opacities can be lysed
under direct vision.
adhesions while a first-year resident assisted with retraction from 8:27 to 8:58 a.
The sample preparation technology covered by the patents provides several key advantages for extracting nucleic acid from a sample, including (1) concentration of the cells in the sample to be lysed
on a solid phase material, such as a filter; and (2) highly effective ultrasonic lysis of the concentrated cells.
Then cells were lysed
in 2 mL HBSS, the lysate was clarified, and a portion of it was used for the first passage.
2], cells were washed with PBS and lysed
with sodium dodecyl sulfate (SDS)--polyacrylamide gel Laemmli sample buffer.
The stained cells were subsequently lysed
with 1X FACS[TM] Lysing Solution and the obtained leukocytes were resuspended in 1 mL of 1 % fixative (Formaldehyde, Electron Microscopy Sciences, Fort Washington, PA) after washing twice with 1X PBS/0.
The filtered samples were washed with 5 mL of water and then lysed
for ~20 s.
In a second study, Barry Komm and Mark Haussler, working at the University of Arizona College of Medicine in Tucson, inclubated lysed
nuclei of sarcoma cells from rat and human bone with highly radioactive estrogen.