Samples for dissolved oxygen were placed in DOB bottles and fixed with 1 mL of manganous
sulfate and 1 mL of potassium iodide.
1) The premix provided per kilogram of diet: 60 mg of iron as iron sulfate, 48 mg of manganese as manganous
oxide, 48 mg of zinc as zinc oxide, 12 mg of copper as copper sulfate, 0.30 mg of iodine as calcium iodate, 0.36 mg of selenium as sodium selenite, Vitamin A, 25,000 IU; Vitamin D, 340,000 IU; Vitamin E, 1,000 IU.
Yamashita, "Oxidation-reduction potential of a paddy soil in situ with special reference to the production of ferrous iron, manganous
manganese and sulfide," Soil Science and Plant Nutrition, vol.
Fridovich, "The scavenging of superoxide radical by manganous
complexes: In vitro," Archives of Biochemistry and Biophysics, vol.
Zhu, "Synthesis and magnetic and catalytic properties of manganous
isonicotinoyl hydrazone complexes," Synthesis and Reactivity in Inorganic, Metal-Organic, and Nano-Metal Chemistry, vol.
([section]) Mineral premix (g TOO g premix): sodium dihydrogen phosphate, 21.5; calcium dihydrogen phosphate, 26.5; calcium carbonate, 10.5; Ca-lactate, 16.5; cobalt chloride, 0.001; cupric sulfate pentahydrate, 0.0625; ferrous sulfate, 1.0; magnesium sulfate heptahydrate, 7.0995; manganous
sulfate monohydrate, 0.1625; potassium iodide, 0.0167; sodium selenite, 0.0025; and zinc sulfate heptahydrate, 3.298.
Calcein molecules released manganous
ions competitive binding to the newly generated pyrophosphate ions from the LAMP reaction, thereby recovering its green fluorescence.
NBRIP (1 L) included 10 g Glucose, 0.5 g Ammonium sulfate, 0.2 g Sodium chloride, 0.1 g Magnesium sulfate, 0.2 g Potassium chloride, 0.5 g Yeast extract, 0.002 g Manganous
sulfate, and 0.002 g Ferrous sulfate, with 2g Calcium phosphate (or aluminum phosphate or iron phosphate) as phosphate source.
(2) Provided per kg of mixed diet: folic acid, 1.5 mg; biotin, 0.15 mg; Fe, 150 mg as ferrous sulfate; Cu, 125 mg as copper sulfate; Zn, 200 mg as zinc oxide; Mn, 30 mg as manganous
oxide; I, 0.3 mg as potassium iodide; and Se, 0.3 mg as selenium selenite.
All the soils received the same planting fertilization, in mg [dm.sup.-3]: 400 of phosphorus (P), 169 of nitrogen (N), 120 of potassium (K), 50 of sulfur (S), 10 of manganese (Mn), 2 of copper (Cu), 0,8 of boron (B), and 0,3 of molybdenum (Mo), in the form of purified monoammonium phosphate (MAP), potassium sulphate, manganous
sulphate, copper sulphate, boric acid, and ammonium molybdate.
After that stirring and cooling to room temperature, 0.01mol manganous
chloride aqueous solution was slowly added dropwise to the vessel of the mixture with stirring.
Bromfield SM, David DJ (1976) Sorption and oxidation of manganous
ions and reduction of manganese oxide by cell suspensions of a manganese oxidizing bacterium.