This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione
, a potent inducer of free radicals, and interleukin 1-[beta] converting enzyme .
Future treatment may also involve CoQ10 supplementation and respiratory chain cofactors, such as, riboflavin, tocopherol (vitamin E), succinate, ascorbate (vitamin C), menadione
, and nicotinamide.
Each kg of premix consisted: pyridoxine, 1 mg; folic acid, 0.4 mg; molybdenum, 0.32 mg; ethoxyquin, 25 mg; choline chloride, 60 mg; dI-[alpha]-tocopherol acetate, 4 mg; iodine, 0.2 mg; thiamine, 0.3 mg; Ca pantothenate, 3 mg; cyanocobalamin, 3 ug; biotin, 0.02 mg; Mn, 15 mg; Zn, 10 mg; iron, 4 mg; Cu, 1 mg; Co, 0.06 mg; Se, 0.02 mg; cholecalciferol, 0.018 mg; trans-retinol, 0.66 mg; menadione
, 0.4 mg; riboflavin, 1.6 mg and niacin, 6 mg.
In the present study, we have identified the antioxidative effect of Y-27632 in TM cells by microarray analysis, an exhaustive investigation of gene expression, and shown that Y-27632 partially suppresses ROS production and cell death induced by menadione
. To the best of our knowledge, this is the first report to show the antioxidant effect of ROCK inhibitor on TM cells.
in the concentration of 200 ppm served as positive control, while distilled water with DMSO 0.1% was used as negative control.
gingivalis were grown anaerobically in brain heart infusion supplemented with 5 [micro]g/mL hemin and 0.5 [micro]g/mL menadione
. Aggregatibacter actinomycetemcomitans was grown anaerobically in Todd Hewitt Broth (OXOID Ltd., Hampshire, UK).
Phenylmethylsulfonyl fluoride (PMSF), ethylenediaminetetraacetic acid (EDTA), 2,4-dinitrophenylhydrazine (DNPH), 5,5'-dithiobis-2-nitrobenzoic acid (DTNB), 2,3,5-triphenyl tetrazolium chloride (TTC), oxidized glutathione (GSSG), NADPH, FeS[O.sub.4] x 7[H.sub.2]O, and menadione
were obtained from Sigma-Aldrich Corporation (USA); diagnostic kits for determination of glucose were from Cormay (tomianki, Poland); N,N,N',N'-tetramethyl ethylenediamine (TEMED) and quercetin were from Reanal (Hungary); peptone and yeast extract were from Fluka (Germany).
nidulans, when it was exposed to six types of stresses, including oxidative stress (menadione
sodium bisulfite (MSB), low and high concentration of [H.sub.2][O.sub.2], t-butylhydroperoxide (tBOOH), diamide) and high-osmolarity stress (NaCl) .
1: Provided (per kilogram of diet): Retinol 3.03 mg; cholecalciferol3 0,10 mg; a-tocopheryl acetate 30 mg; menadione
5.0 mg; thiamine 3 mg; riboflavin 6 mg; pyridoxine hydrochloride 5 mg; cyanocobalamin 0.03 mg; niacin 30 mg; biotin 0.1 mg; calcium D-pantotenat 12.0 mg; folic acid 1.0 mg; colin chloride 400 mg.
Vitamin K exists as vitamin K1 (phylloquinone), which is abundant in many vegetables; vitamin K2 (menaquinone), which the body produces in the digestive tract and which is provided by some animal products; and vitamin K3, the synthetic form known as menadione
Ingredient Amount (%) Yellow corn 30.0 Barley 10.0 Soybean meal 38.0 Sunflower meal 6.0 Wheat bran 10.0 Molasses 3.0 Limestone 1.5 Salt 0.8 DL-Methionine 0.3 Dicalcium phosphate 0.2 Vitamin and mineral premix (1) 0.2 Analyses (%) CP 24.3 Ether extract 3.4 Crude cellulose 6.9 Ash 8.1 Ca 1.3 P 0.9 (1) The vitamin-mineral premix provides the following (per kg): all -trans-retinyl acetate, 1.8 mg; cholecalciferol, 0.025 mg; all-rac-a -tocopherol acetate, 12,5 mg; menadione
sodium bisulfate), 1.1 mg; riboflavin, 4.4 mg; thiamine (thiamine mononitrate), 1.1 mg; vitamin B-6, 2.2 mg; niacin, 35 mg; Ca-pantothenate, 10 mg; vitamin B-12, 0.02 mg; folic acid, 0.55 mg; d-biotin, 0.1 mg.