The determination of the minimum inhibitory concentration to heavy metals has revealed a considerable resistance of the transconjugant strains to mercury chloride, which can withstand concentrations up to 1250 [micro]g /ml, in contrast to the metal-sensitive recipient strain (MIC = 200 [micro]g /ml).
fluvialis was observed by growth carve at different concentrations of mercury chloride. The highest mercury-remediation rate (60%) was found at a lower mercury concentration of 100 [micro]g/ml after 42 h of incubation.
The explants were surface sterilized in 70% ethanol for 1 min, followed by sodium hypochlorite (NaOCl) 5% (v/v) plus 0.01% Tween-20[R] for 10 min and mercury chloride (Hg[Cl.sub.2]) (0.1 and 0.2% w/v) for 2 to 4 min under constant agitation.
Some settlers kept pills called "blue mass" in their medicine cabinets to be used like aspirin at the first hint of illness, or if things got bad quickly, doctors administered calomel, a pure, fast-acting form of mercury chloride.