The protein aggregates or large particles were formed by heavy meromyosin
and light meromyosin
via changing their conformations .
Myosin II presents two fragments; light meromyosin
(LMM), which corresponds to two thirds of the tail and is responsible for the self-assembly of protein dimers, and heavy meromyosin
(HMM), that is divided in two subfragments; the subfragment 1 (S1), which includes the motor domain, is consisted of globular heads, and the subfragment 2 (S2), which corresponds to the proximal third of the tail and acts as flexible connection between S1 and LMM (Craig & Woodhead, 2006).
After 24 h marinating the 200 KDa polypeptides (heavy meromyosin
) were identified in all samples (Figure 3).
Expression and purification of full-length of Physarum myosin, short-tailed heavy meromyosin
, and Physarum myosin with mutant Ca-binding light chain.
Squid mantle muscle contains endogenous metalloproteinases that selectively degrade myosin molecules into heavy meromyosin
(HMM) and light meromyosin
(LMM) (Yoshioka et al., 2005).
The [beta]-myosin heavy chain is divided into three regions: the subfragment 1 (S1), subfragment 2 (S2) and light meromyosin
Lin, "Opposite effects of overexpressed myosin Va or heavy meromyosin
Va on vesicle distribution, cytoskeleton organization, and cell motility in nonmuscle cells," Cell Motility and the Cytoskeleton, vol.
The S1 meromyosin
fragment will be fixed at this level, resulting in the change of the angle between the 2 myosin parts, namely its head and neck.