In the 1970s, we demonstrated spasm of an epicardial coronary artery or coronary spasm during an attack of variant angina systematically induced by methacholine
or exercise in the early morning.
In vivo airway responsiveness of the mice to methacholine
was measured within 24 h after the last OVA challenge using whole-body plethysmography (Buxco Electronics Inc., USA).
Patients were excluded if they were using long-term oral steroids, pregnant or breastfeeding, unable to tolerate spirometry or methacholine
challenges, or had a history of more than 10 pack-years of smoking.
In brief, the mice were exposed to nebulized PBS for 3 min to establish baseline sRaw values, followed by exposure to increasing concentrations of nebulized methacholine
(6.25-25 mg/mL; Sigma, USA) using an Aerosonic ultrasonic nebulizer.
Relationships of bronchial responsiveness assessed by methacholine
to serum IgE, lung function, symptoms, and diagnoses in 11-year-old New Zealand children.
 In a separate study in a highly polluted area in the south of Durban, which included 248 school-aged participants from grades 3 to 7, 52% had asthma of any severity, 11% had moderate-to-severe persistent asthma, and 21% had marked airways hyperreactivity on methacholine
Happel recommends moving on to bronchial hyperreactivity testing via a methacholine
the dosimeter is intended to administer the test substance in the methacholine
inhalation prophylaxis and to administer the pentacarina treatments in the lohja medical care area.
Assess of Airway Hyperresponsiveness to Methacholine
. Airway hyperresponsiveness (AHR) of mice to methacholine
was measured before necropsy.
Mice were aerosolized with phosphate-buffered saline (PBS) and methacholine
(3 and 6 m[micro]g/mL) through an inlet of the individual chambers for 2.5 min, and Penh readings were recorded for 5 min following each nebulization.
After the last challenge, airway resistance of mice was analyzed by stimulating with normal saline and increasing concentrations of methacholine
(Sigma-Aldrich; 10, 30, and 100 mg/ml) for 30 seconds.
Guidelines for methacholine
and exercise challenge testing - 1999.