On biopsy, the cell wall will stain with either methenamine
silver or periodic acid-Schiff, and the capsule will stain with mucicarmine or Alcian blue.
Special stains were most commonly used: periodic acid-Schiff (PAS) in 155, Jones methenamine
silver in 13, and trichrome in 15.
Silver Stain (Ventana Medical Systems, Inc.
This can be done by demonstrating fungal hyphae in viable tissue with aid of special stains like Gomori Methenamine
Silver (GMS) and Periodic Acid Schiff (PAS).(3) Fungal cultures are positive in only 33% of cases and are time consuming.
Neither fungal organisms nor malignancy was observed on periodic acid-Schiff (PAS) and Gomori methenamine
silver (GMS) staining.
Histology revealed dermal and subcutaneous abscesses, No fungal elements were identified using acid-fast bacilli and Gomori's methenamine
silver stains, but acid-fast bacteria were identified in the abscess cavities.
, either as the mandelate or hippurate salt, is an old drug that is now rarely used in pregnancy.
The minimum serum transaminase value was 0.40 U/L and maximum serum transami- nase was 55.0 U/L with a mean S.D of serum trans- aminase being 27.74 15.61 U/L.All the biopsies in addition to the Haematoxylin eosin stain with Periodic acid Schiff's reaction (PAS) to view the mesangial matrix potential expansion in mat- rix mesangial cells alterations in basement memb- rane and vessels Congo red to visualize amyloid depo- sits Masson's trichrome to see the extent of fibrosis and Jones Methenamine
silver stain for the detection of change of GBM.
Though H&E does have the capacity to stain Aspergillus, it is limited in its identification of other fungi and is frequently supplemented by additional stains such as Gomori's methenamine
siliver (GMS) and periodic acid-Schiff (PAS) so that fungal morphology can be better highlighted.
Periodic acid-Schiff and Gomori methenamine
silver will facilitate visualization of the causative microorganisms in blastomycosis, coccidiomycosis, and paracoccidiomycosis, whereas specific histochemical stains are typically not needed for visualizing the diverse pigmented fungal organisms that cause phaeohyphomycosis, but periodic acid-Schiff and Gomori methenamine
silver may still be useful.
A section of pericloacal skin and subcutis containing the suspect granuloma was examined, and the core of the inflammatory nodule contained caseous cellular debris admixed with many large gram-positive rods that were acid-fast negative, black with Grocott's methenamine
silver (GMS) stain, and blue with Giemsa stain.
The staining currently automated are: Massons~s trichrome (or Gomori~s trichrome), Grocott~s Methenamine
Silver (or Gomori~s Methenamine
Silver), Giemsa, Gordon and Sweet (or Gomori and Snook), Congo Red, Jones Methenamine
Silver (with H&E contrast colour), Gram, PAS + diastase (2 kit), Perls Prussian Blue, Alcian Blue (pH 2,5), Ziehl-Neelsen, Elastin (Modified Weigert~s Resorcin Fuchsin, Verhoeff~s elastic tissue).