The phrase "transgenic rescue with wild-type and mutant construct" means that researchers can microinject
various plasmids to see whether the sequences can rescue the mutant phenotypes to understand whether some genetic variants retain wild-type function or not.
PALM Robot-CombiSystem combines the cutting ability of the Robot-MicroBeam with the manipulation capabilities of the Robot-MicroTweezers, allowing a user to handle a small object by optical tweezers and simultaneously cut, microsurgically process, or microinject
the trapped specimen.
But straightforward microinjection won't work with plant cells, because "it's pretty hard to microinject
through a tough cell wall," says Michael Berns, director of the Beckman Institute, who has done extensive research on laser microsurgery.