Thus, we herein investigated if functional blockade of Egr3 hinders meiotic maturation of oocytes by microinjecting
dominant-negative Egr3 complementary RNA (cRNA) and small interfering RNA (siRNA) specific to Egr3.
18) Foreign genes are introduced into a host organism in a number of ways, including (a) particle gun blasting of tiny particles of alien DNA into the host cells; (b) infecting the host with a bacterium modified to carry the donor into the host cells; (c) microinjecting
the DNA into plant cells directly; and (d) chemical or electrical treatments designed to trigger the host's direct intake of foreign DNA.
Thus, its simplicity and non invasiveness may provide a new avenue for microinjecting
various substances into a wide range of living tissues.
These QDs were not functionalized, which precluded specific labeling; however, on the basis of our observations and discussions, these researchers took the approach of microinjecting
QD-containing micelles into cells.
In combining this variety of qualitative data, obtained from a variety of methods, as a single dataset, it was envisaged that such an approach would contribute to a wider understanding of public injecting in terms of the interrelationship between local structures within the macroenvironment (relating to policy, practice, and enforcement), individual agency (IDU), and of specific microinjecting
environments located in the field.
1990) first tested this idea by microinjecting
CRE oligonucleotides into sensory neurons co-cultured with motor neurons.
A standard technique for getting genes into animal cells is by microinjecting
them with a small needle.
At present, the primary method used to create transgenic animals -- which are used in disease research and to improve livestock -- involves microinjecting
DNA into embryos.
The method of microinjecting
foreign DNA into the pronuclei has been considered as a practical means for the production of transgenic livestock, although the achievement of transgenesis is less than 1% in livestock (Wall, 1996).
This preferential coalignment was disrupted by microinjecting
cells with antibodies to detyrosinated tubulin, but not with antibodies to tyrosinated tubulin.
The experiments were done by microinjecting
genetic material coding for BLG into fertilized mouse eggs that were then reimplanted into "surrogate' female mice.