After step-wise tissue processing, embedding, blocking and microtomy
of the available core needle biopsy specimens, haematoxylin and eosin (H and E) stained sections were prepared for light microscopic examination in each case.
The expansion of the paraffin-embedded material after microtomy
(usually in a water bath) is also not uniform throughout the preparation of the histological slides and may introduce a bias when morphometry is made in the slides (Mandarim-de-Lacerda, 1987).
Tissues were processed in automatic tissue processor and embedded in molten paraffin wax to make tissue blocks for microtomy
Spherocrystals found in the cytoplasm of absorptive cells are easily removed during the preparation for microtomy
, resulting in empty sites (Figure 4A, B).
These sections were then routinely processed according to the standard procedures for paraffin embedding, microtomy
and staining with Haematoxylin and Eosin stains.
Paraffin sections of 3-5 microns thick were prepared from the paraffin blocks by microtomy
followed by routine staining with hematoxylin and eosin .
Accessioning, gross cassette verification, tissue processing, embedding, microtomy
, order entry, primary staining, advanced staining, case assembly, pathologist review and archive are the various processes of tissue diagnostics.
Fixation, Preparation of tissues for microtomy
, General outline of the theory and practice of staining and general staining procedures.
For example, at Henry Ford Hospital in Detroit, Michigan, pathologists reported a 62% decrease of overall misidentification case rate, 92% decrease in slide misidentification defects, and 125% increased technical throughput at their microtomy
workstations after bar codes were introduced.
UVA depth profiling is now routinely done using slab microtomy
sectioning at ~7-10 1.
Theory of microtomy
artefacts in arthropod cuticle.
Each slide contained four sections of the same fragment, which was established a distance of 30 [micro]m between the sections during microtomy