Longitudinal sections of each mice skin specimen (about 5 mm diameter and 5 [micro]m thickness) were prepared by microtomy
and stained with hematoxylin and eosin (H and E) dye for histological examination.
After processing, paraffin blocks were prepared at each institution and sent to Memorial Sloan Kettering Cancer Center (New York, New York) for microtomy
and slide preparation.
and staining of the tissues: Formalin-fixed tissues were washed with water and dehydrated with ethanol solution in ascending order.
After the fixation and permanence in alcohol 70%, a procedure for the inclusion of the material in paraffin was carried out, as well as the microtomy
in the rotary microtome Microm[R] HM 325, which performed transversal cuts 6mm thick.
(4) Removal of the need for a costly microtome and other steps involved in microtomy
: making longitudinal section of the tooth as thin as 57 [micro]m by grinding on a set of sandpapers with hands without using a microtome is the main key feature of this technique.
They were then serially sectioned to 5 [micro]m thickness using microtomy
, stained with Hematoxylin and Eosin (H&E) stain and were fixed on microscopic slides using DPX mountant.
After step-wise grossing, tissue processing, embedding, blocking and microtomy
, haematoxylin and eosin (H and E) stained sections were prepared for light microscopic examination in each case.
can be performed using disposable blades as per routine protocol.
Various experimental methods, which mainly include the mercury intrusion method , low-temperature nitrogen adsorption method , microtomy
method , and computed tomography (CT) , have been adopted by many scholars for investigating coal's microstructure.
It would be of great interest to extend this characterization to Kevlar fibers that (i) constitute new Kevlar classes with unique processing conditions and (ii) are not subjected to microtomy
, which can yield significant surface artifacts .
Tissues were processed in automatic tissue processor and embedded in molten paraffin wax to make tissue blocks for microtomy
. Tissue sections of 5um thickness were obtained to stain with haematoxylin and eosin stain (HandE) and aldehyde fuchsin stain for beta cells and immunohistochemical (IHC) stain using Bandeiraeasimplicifolia (BS)-1 lectinto stain intra-islet capillaries.