Finally, the nonmigratory cells were scraped off and washed gently in PBS, the migratory cells
on the bottom surface of the transwell membrane were fixed in cold methanol for 30 min and underwent crystal violet staining, then photographed under a digital microscope (Olympus BX51; Olympus, Tokyo, Japan).
Biologists at the California Institute of Technology (Caltech) led by an Indian origin researcher have now found that neural-crest stem cells-multipotent, migratory cells
unique to vertebrates that give rise to many structures in the body such as facial bones and smooth muscle-also play a key role in building olfactory sensory neurons in the nose.
Having overcome cell adhesion, migratory cells
must penetrate the basement membrane.