The supernatant from the liquid cultures was filtered through a 0.2-[micro]m filtration unit (Millepore
Express PLUS) to remove any remaining cells, and the organic compounds were separated by liquid-liquid partitioning with ethyl acetate and butanol, sequentially.
Briefly, the brain sections were washed of cryoprotectant and placed into primary antibody solutions for GFAP (AB5804, 1:4000 dilution; Millepore
, Billerica, MA) or IBA-1 (016-20001, 1:5000; Wako Chemicals USA, Richmond, VA) for 24 hr.
White wines get finished with cold stabilization in the Transtores and sterile filtration with a Millepore
Seawater used in the experimental chambers was collected from outside the bay on the fore reef, passed through activated charcoal and millepore
Sequences belonging to the barnacle Wanella millepore
were found in 10 different lesion samples, but only from 2 locations.
[their] fallen trees in every possible stage of decay," (11) but she also could see and name, as Dickens apparently could not, individual trees, shrubs, herbs, and flowers: The beech, the chestnut, the tulip tree, the palmetto, pawpaw, the pennyroyal, even the millepore
. And she knew birds and named them.
Pollution study of Hilo Bay using the millepore
This protocol involved sequentially cleaning the labware in a series of baths in solutions (1 week each) and rinses (five per solution) in a three-step order: a) we used a detergent solution (Micro; American Scientific Products, McGraw Park, IL) and deionized water rinses; b) we used a 6-N HCl (reagent grade) solution and ultrapure (Milli-Q; Millepore
Corp., Bedfor, MA; 18 M[Omega]/cm) water rinses; and c) we used a 7.5-N [HNO.sub.3] (trace metal grade) solution and ultrapure water rinses.
The project HydroReef aims to characterize biodiversity and metapopulation dynamics of millepores
through a multidisciplinary approach involving DNA barcoding, phylogeny, comparative phylogeography and population genetics on various spatiotemporal scales.