According to the manufacturer, both presentations have a composition based on calcium silicates, zirconium oxide, tantalum oxide and monobasic
potassium dihydrogen orthophosphate buffer (20 mM; pH 3.5) and acetonitrile were used as mobile phase (65:35 v/v).
Components Elements Concentration mg.[L.sup.-1] MS Ammonium nitrate 1650 Macronutrients (N[H.sub.4]N[O.sub.3]) Potassium nitrate (KN[O.sub.3]) 1900 Calcium chloride 440 (Ca[Cl.sub.2].2[H.sub.2]O) Magnesium sulfate 370 (MgSO4.7[H.sub.2]O) Potassium phosphate monobasic
170 (K[H.sub.2]P[O.sub.4]) MS Manganese sulfate 22.3 Micronutrients (MnS[O.sub.4].[H.sub.2]O) Zinc sulfate 8.6 (ZnS[O.sub.4].7[H.sub.2]O) Boric acid ([H.sub.3]B[O.sub.3]) 6.2 Potassium iodide (KI) 0.83 Sodium molybdate 0.25 ([Na.sub.2]Mo[O.sub.4].
All critical amino acid residues associated with influenza A virus replication and entry (4,5) were conserved between the Brazil and the Peru HL18NL11 strains, including the HA monobasic
cleavage site motif PIKETR/GLF (5).
The concentration of phenols is also limited--the monobasic
phenols in the effluent should not exceed 0.1 mg/L .
Reagents with an analytical grade were used in the determinations, that comprise sodium hydroxide (Vetec, Brazil), sodium phosphate monobasic
(Biotec, Brazil), ethanol (Biotec, Brazil) nitric acid (Biotec, Brazil), commercial humic acid (Sigma Aldrich, USA) and standard of the sulfate of streptomycin (Sigma Aldrich, USA).
But it has certain drawbacks, including long setting times, difficulty with manipulation, limited resistance to washout before setting, and the possibility of staining tooth structure.10 Endosequence BC Root Repair Material (ERRM; Brasseler USA, Savannah, GA, USA) is a combination of calcium silicates, zirconium oxide, tantalum oxide, calcium phosphate monobasic
and filler agents.
The equimolar amounts of 1,2-phenylenediamine and monobasic
acids (formic acid, acetic acid and propionic acid separately) were refluxed for 4 hours in 4N HCl.
Each follicle was placed immediately in a container with PBSE medium comprised of 0.072 M dibasic potassium phosphate, 0.046 M sodium glutamate, 0.027 M fructose, 0.031 M sodium acetate, 0.016 M N-Tris (hydroxymethyl/ aminomethane), 0.0019 M potassium citrate, 0.0047 M monobasic
potassium phosphate, and 0.0027 M tetrahydrate magnesium chloride.
A nutrient cultivation solution was added to these containers, consisting of 240 mL of (1 mol [L.sup.-1]) solution of [(N[H.sub.4]).sub.2]C[O.sub.3] (Ammonium Carbonate) and 90 mL of (1 mol [L.sup.-1]) solution of [K.sub.2]HP[O.sub.4] (Dibasic Potassium Phosphate), as well as K[H.sub.2]P[O.sub.4] (Monobasic
Phosphate) for pH buffering between 6.5 and 7.0, similar to the pH of the collecting area.
The chemicals and reagents used to perform the experiments were the following: monobasic
ammonium phosphate (FARMITALIA CAROERBA, Italy), sodium hydroxide (BDH limited, Poole, England), HPLC grade acetonitrile (BDH Laboratory Supplies, England), 85% phosphoric acid (Riedel-de Haen, Germany), potassium phosphate monobasic
(Fisher Scientific, USA), HPLC grade methanol (Park Scientific Limited, UK), and distilled water.