In the urinary tract, CaP may be present in the form of hydroxyapatite, carbonate apatite, or brushite (calcium monohydrogen
phosphate dihydrate, CaHPO4.2H2O).
Phosphate buffered saline (PBS) was obtained from Gibco, Karlsruhe, Germany; methanol, ethanol, and sodium bicarbonate were obtained from Fisher Scientific, UK; acetic acid was obtained from BDH, Poole, England; trichloroacetic acid and haematoxylin and eosin Y were obtained from Abbey Color, Philadelphia, USA; xylene, formaldehyde, thiobarbituric acid, EDTA, monopotassium dihydrogen phosphate, dipotassium monohydrogen
phosphate, monosodium phosphate, disodium phosphate, [lambda]-carrageenan, o-dianisidine dihydrochloride, and Evans blue dye were obtained from Sigma-Aldrich, St.
The most prominent phosphate band is detected at ~961 [cm.sup.-1], whereby the exact position is sensitive to monohydrogen
phosphate (HP[O.sub.4]) .
[O.sup.-] hydrogen bonding through conformational analysis of 4-Amino-4-oxobutanoate (succinamate) and monohydrogen
1, 4-butanoate (monohydrogen
succinate) anions," Journal of Physical Chemistry A, vol.
Prepare a mixture of monobasic dihydrogen phosphate (11.49 gr) and dibasic monohydrogen
phosphate (2.16 gr).
Succinicacid, potassium dihydrogen and monohydrogen
phosphate, glycine, pyrogallol, hydrogen peroxide, trichloroacetic acid (TCA) and ethylenediaminetetra-acetic acid (EDTA), sulphosalicylic acid, and thiobarbituric acid (TBA) were purchased from BDH, England.
Additional ingredients of the eye drops were sodium chloride, sodium dihydrogen phosphate dihydrate, sodium monohydrogen
phosphate dihydrate, and water.
Agarwal, "Anion recognition through modified calixarenes: a highly selective sensor for monohydrogen
phosphate," Analytica Chimica Acta, vol.538, pp.213-218, 2005.
Lecithin 200, 100p were from Locus Meyer industry, Germany, lecithin 170 was from Degussa, Germany, n-butanol, isopropyl alcohol, n-propanol, potassium monohydrogen
phosphate ([K.sub.2]HP[O.sub.4]), NaOH, and sodium lauryl sulphate (SLS) were from Merck Chemical Company, Germany, cellulose membrane from Biocin industry, Belgium, isopropyl myristate from Aldrich company, Germany, and metoprolol was from Alborz Darou, Iran, as a gift sample.
Sulphamethazine, sulfadiazine and monohydrogen
phosphate, sodium dihydrogen phosphate, were purchased from Sigma, deionized distilled water was obtained from Adventec (GS-590, distillary and CPW-200), Japan from Central High Tech Lab., University of Agriculture, Faisalabad (UAF).
(29) Brushite (calcium monohydrogen
phosphate) stones comprise about a quarter of calcium phosphate stones.
The reagents used were analytical grade and included sodium hydroxide (NaOH), disodium monohydrogen
phosphate [(Na.sub.2][HPO.sub.4].2[H.sub.2]O),potassiumdihydrogenmonophosphate ([KH.sub.2][PO.sub.4]), perchloric acid (HCl[O.sub.4]), methanol, distilled water and pyrazinamide standard (Sigma Chemical Co., St Louis, Mo).