Finally, a mutant screen of mutagenized
gai plants is already under-way within the group.
Plaques formed by mutagenized
viral populations have elevated coinfection frequencies.
In this connection, wild bacterial strain Streptococcus equisimilis was mutagenized
using ethidium bromide and UV radiation.
The developed method is applied for comparative analysis of glycosylation patterns of biosimilar rAbs as well as a mutagenized
As an alternative, variants with relaxed specificities within the PAM could be evolved , and the predicted PI-domain from SaCas9 was randomly mutagenized
and tested for PAM specificity, resulting in a Cas9 variant called KKH (E782K/N968K/R1015H) which showed the same DNA-cleavage specificity when compared to its wild-type counterpart (5'-NNGRRT-3').
TILLING (Targeting Induced Local Lesions IN Genomes) and EcoTILLING which screen mutagenized
and natural populations, respectively, have been instrumental in identifying mutants of interest within a short period of time .
Screening for Cocaine Sensitivity in Mutagenized
conidia were grown in liquid minimal medium having 2% xylose and 0.5% (w/v) 2-deoxyglucose (2-DG).
Then, we mutagenized
the transgenic animals and searched for "green" worms by using a fluorescence-activated worm sorter.
We have demonstrated that BaP exposure during fetal development causes severe somatic and germ cell consequences in F1 animals and that tissues originating from all three germ layers, as well as the germ cells themselves, are highly mutagenized
in adult offspring.
For raffinose synthase (MU-RS2-1 and MU-RS2-2) assays, mutant sources (PI 200508 and mutagenized
line 397) and artificial heterozygotes were tested along with a wild-type genotype, cultivar Williams 82.
In particular, mutagenized
KBM7 cells were treated with Escherichia coli-derived CDTs and resistant clones were isolated, leading to identification of insertions in the sphingomyelin synthase 1 and the putative G protein-coupled receptor TMEM181, suggesting that this molecule may serve as a surface receptor for the toxin [43,44].