Neutrophils derived from bone marrow hematopoietic stem cells, which undergoes multiple developmental stages, such as myeloblast, premyelocyte, myelocyte
, metagranulocyte, band neutrophils, and eventually develop into mature neutrophils.
No HJBLI found in granulocytes 6 Deletions 7q No HJBLI found in granulocytes and 17p 7 Trisomy 19 HJBLI identified in <5% of neutrophil population 8 5q deletion, HJBLI identified in <5% of monosomy 7 and neutrophil population trisomy 8 9 20q deletion HJBLI identified in <5% of neutrophil population 10 5q and 17p deletion HJBLI identified in <5% of neutrophil population and < 1% of myelocyte
precursor population 11 5q deletion HJBLI identified in < 5% of neutrophil population Abbreviations: AML, acute myeloid leukemia; HJBLI, Howell-Jolly body-like inclusion; MDS, myelodysplastic syndrome; MDS-EB, myelodysplastic syndrome with excess blasts; MPN, myeloproliferative neoplasm; RBC, red blood cell; WHO, World Health Organization.
Congenital neutropenia is a disease that develops in bone marrow due to the arrest in promyelocyte or myelocyte
maturation and differentiation (1).
Megakaryocytes count was performed under 10x magnification whereas the other cell counts (rubriblite, prokaryotic, rubricito, metarrubic, myeloblast, promyelocyte, metamielocyte, myelocyte
, rodent, eosinophils, basophils, neutrophil, lymphocyte, plasma cell, and monocyte counts) were done using the 100x objective with oil immersion.
The PB findings were as follows: hemoglobin 79 g/L; WBC 33.5 x 10[sup]9/L with 3% promyelocyte, 15% myelocyte
, 19% metamyelocytes, 48% neutrophils, 4% lymphocytes, and 11% monocytes; and platelet count 81 x 10[sup]9/L.
Immature neutrophils include promyelocyte myelocyte
, metamyelocyte and band form.
Severe congenital neutropenia appeared unlikely as the bone marrow did not show 'maturation arrest' at the myelocyte
stage of development, the promyelocytes were not increased nor showed dysplastic morphology such as large size, atypical nuclei or vacuolated cytoplasm.
Maturation stages in the bone marrow are as follows: stem cell, myeloblast, promyelocyte, myelocyte
, metamyelocyte, band and neutrophil.
In order to quantify immune cell densities (cells/ mm2) in pre-neoplastic (DCIS), neoplastic (IDC) and control tissues, College of American Pathologists (CAP) guidelines were used.16 Immune cells were identified and grouped as either myelocyte
cells or lymphoid cells based on morphology as observed via microscopy using H and E stained slides.
Tmem200a was a transmembrane protein which might inhibit overgrowth of myelocyte
We also measured and compared the level of N6AMT1 mRNA in several cell lines, including 293 (human embryonic kidney cells), HeLa, UROtsa, and HL60 (human pro myelocyte
Tissue examination showed granulocytic cells in different stages of maturation, but with a distinct and prominent shift toward immature forms at the promyelocyte and myelocyte