The endothelium-dependent vasorelaxation was measured using the 620M myograph
system (DMT, Denmark) as described previously (9).
These ex vivo vascular rings were drawn by two stainless steel needles which were located at the bath of DMT myograph
(ADinstruments, Australia) to achieve 1 g tension and maintained for 1 h.
Isometric force generation was recorded with a Multi Myograph
System (Danish Myo Technology).
TA muscles were fastened with vicryl into the myograph
chambers (DMT, Denmark) and allowed to equilibrate under 15 mN (1.5 g) for 20 min, adjusting the tension periodically and replacing Krebs solution every 5 min.
Individual arterial ring segments of 2 mm length were mounted in a four channel small vessel wire myograph
(model 610M Danish Myotech, Denmark).
A segment (2 mm in length) of the mesentery was carefully cleared of adherent tissue and mounted in Krebs buffer at 37[degrees]C in a small vessel myograph
(model 400A, Danish Myotechnology, Denmark).
Fresh or incubated segments were immersed in a temperature-controlled (37[degrees]C) myograph
system (Organ Bath Model 700M0, J.P.
Arterial rings were then mounted on multichannel isometric myograph
, bathed in Krebs-Henseleit solution at 37[degrees]C, bubbled with 95% [O.sub.2]-5% C[O.sub.2], and connected to a force transducer (Pioden controls Ltd, Canterbury, UK for aortic rings; Danish Myo Technology; Aarhus, Danemark, for mesenteric artery rings).
Kassab, "Assessment of endothelial function of large, medium, and small vessels: A unified myograph
," American Journal of Physiology--Heart and Circulatory Physiology, vol.
Detrusor strips were mounted on a myograph
system (Danish Myo Technology Model 800MS, Denmark) with carbogen-aerated Krebs' solution at 37[degrees]C.
Organ chambers (Multi Myograph
System, Danish Myo Technology A/S) were filled with (37[degrees]C) Krebs solution containing (in mmol/L) 119.0 NaCl, 4.7 KCl, 2.5 Ca[Cl.sub.2], 1.0 Mg[Cl.sub.2], 25.0 NaHC[O.sub.3], 1.2 K[H.sub.2]P[O.sub.4], and 11.0 D-glucose.
When the number of arterial segments was more than one in an individual, the average was used for that individual in myograph