Nestin is a widely used NSC marker, and its expression initiates from the formation of neurula
To study the ontogenetic expression profiles, fertilized embryos and larvae from different developmental stages (fertilized, 2-cell, multicellular, midgastrula, neurula
, muscular contraction, pre-hatching, post-hatching, seven days post-hatch) were collected following natural spawning of the brood stock.
Development from hatched neurula
embryos through early larvae
A recent study showed that expression of notochord-specific genes with multiple and single functional Brachyury binding sites begins around the early gastrula and neural plate stages, respectively, and expression of indirect targets of Brachyury begins around the early neurula
observaron las 3 hojas germinativas y la neurula
alrededor de los 12 y 14 dias postcopula, respectivamente.
The early neurula
is finished with formation of the expressed nervous plate and a cavity of a primary gut.
In the current study, messenger RNA (mRNA) will be obtained from cardiac tissue of embryos that have been treated with 100nm of ATRA in the neurula
stage of development.
Twenty-four hours after mating, embryos at the early-cleavage, blastula, gastrula, neurula
, and tadpole stages were collected, preserved in Bouin's fixative, sputter coated with a mixture of platinum and gold, and observed using a scanning electron microscope (S-2460N, Hitachi).
In my experiment, when the egg became flattened, two neural tubes occurred in the neurula
, and then a twin embryo was produced.
Their cell division is finished by the neurula
stage (Miyamoto and Crowther, 1985; Munro and Odell, 2002a; Jiang et al.
A twofold destructive/proliferative action of injury itself is fundamental in the organizative phenomena during the late blastula, gastrula and neurula
stages, as shown in the Bufo arenarum species when specimens are submitted to experimental exogenous injury.
Further studies will compare the effects of both all-trans and 9-cis retinoic acid on early neurula