However, an intravascular contrast injection (
nondiffusible), while unable to absolutely quantify CBF, can infer it by using the central volume principle [12,13] (cerebral blood flow calculated as a ratio between blood volume in a given volume of tissue and the average time for the tracer to pass through this volume).
[16] Progesterone with its half-life of 30 min causes proportionate fluctuations of diffusible ionic calcium to its
nondiffusible protein bound form, as an immediate effect.
Since then, numerous imaging techniques have been developed and applied to evaluate brain function [27], using different tracers (diffusible or
nondiffusible, endogenous or exogenous), with different technical requirements.
Over the last decades, a vast repertoire of cryoprotectants has been discovered that could be classified into two main categories, namely, diffusible (intracellular agents) and
nondiffusible (extracellular agents).
The colonies grown were around 2 to 3 mm in diameter, convex, nonlactose fermenting, round, glistening, and smooth and produced violet
nondiffusible pigment (Figure 1).
Haptotaxis is defined as a directed migratory response of cells to gradient of fixed
nondiffusible chemicals.
It produces a
nondiffusible red pigment called prodigiosin.
9: Drug susceptibility test on Mueller-Hinton agar with Disk diffusion method also shows
nondiffusible red pigmentation and the following sensitivity pattern:
All of them produced
nondiffusible brown pigments in agar medium, showed motile cells, formed cysts in butanol-containing medium, and showed no fluorescent pigments under UV light (data not shown).
This results in a decrease in blood volume and, hence, an increase in the concentration of anything left behind, i.e., large protein-bound
nondiffusible analytes and cells.