Recombinant lentivirus vectors are widely used in preclinical gene therapy development, including vascular diseases, diabetes mellitus, rheumatoid arthritis and different cancers, etc, characterized by an efficient infection in both dividing and nondividing
cells, and a long-term gene expression.
Ki-67 is a non-histone protein that is usually found in all the phases of cell cycle (G1, S, G2 and mitosis) while it is absent in nondividing
Another advantage is that this technique can be used in non-dividing cells, which means that it could enable gene therapies that focus on nondividing
adult cells, such as cells of the eye, brain, or heart.
UL39 has been associated with neurovirulence in HSV (the ability to replicate in nondividing
nerve cells) and is one of several genes deleted from prototype HSV gene delivery vectors .
Only at the highest concentrations of GO-N[H.sub.2] and pristine GO particles (20 and 50 [micro]g/ml) an increased number of large, nondividing
cells were detected suggesting a weak cytotoxic effect.
Because deletion of the RL1 gene and the [[gamma].sub.1]34.5 gene, respectively, both which encode virulence factors and the herpes virus vectors HSV1716 and HSV-1 M002, is unable to replicate in nondividing
cells, and preferentially infect, replicate in, and lyse rapidly dividing cells such as tumor cells [70, 71].
As a control for nondividing
cells, fibroblasts were stained with CellTrace and analyzed before seeding.
Suzuki and colleagues developed a novel strategy called homology-independent targeted integration (HITI), which allows for targeted NHEJ knock-in in nondividing
cells, such as the photoreceptors .
Gallay et al., "In vivo gene delivery and stable transduction of nondividing
cells by a lentiviral vector," Science, vol.
Immunotoxins exert the function of cell killing by inhibiting protein synthesis, which is toxic to both dividing and nondividing
cells.[sup] In this article, we summarize the properties of the commonly used immunotoxins and their application in recent preclinical and clinical studies in lung cancer.
As a result, the karyotype in some cases may be noninformative when the neoplastic cells are scant or nondividing
. The other limitation of conventional cytogenetics is the inability to detect genotypic changes shorter than 10 Mb in length.
ARPE-19 cell line is commonly used in molecular research on AMD, even though it is a heterogeneous cell population, including dividing and nondividing