To facilitate the examination of the seven SNPs simultaneously, each SNP-specific primer was designed to be a different length by the addition of variable lengths of nonhomologous
d(GACT) polynucleotide tails to the 5' end of the primer.
This bacterial system has emerged as an effective tool for gene targeting through nonhomologous
end joining (NHEJ); however, it has been reported to be inefficient for precise editing of genomic sequences.
Most likely, formation of the Hp 2 allele is the result of a breakage and reunion event at nonhomologous
positions within the fourth and second introns of two Hp 1 genes.
Moreover, the same semiquantitative method showed that the prevalence of pulmonary HIF-1[alpha]+ cells (Figures 5(a)-5(d)), other hypoxia-inducible biomarkers, and Ku-70+ cells (Figures 5(e)-5(h)), an index of DNA repair in nonhomologous
end joining pathway in the medial layer of large PA, was similar to that of TRPC1+ cells among the three groups.
This deletion could be the consequence of nonhomologous
recombination, favored by the possible topoisomerase I recognition sites [(G/C)(A/T)T] (12) or PyTT sites (13) that lie in the close vicinity of the breakpoints.
Furthermore, we also observed that the mixing of control and mutant DNA samples and an extra denaturing/renaturing step increases the chances of HDX formation between the nonhomologous
DNA strands during cross-annealing, making them more apparent and easily visualized with reduced electrophoresis time.
nt, and a 15-nt sequence homologous to the target.
By use of a nonhomologous
internal standard, we eliminated heteroduplex formation during competitive RT-PCR, and we demonstrate that the heterologous RNA standard used in our study and EGFR mRNA in the sample have equal amplification efficiency.
Taking into account the fact that studies have reported a c-erbB-2 overexpression in breast cancer specimens despite the lack of gene amplification [26-28], we decided to develop an assay for quantifying c-erbB-2 gene expression by competitive RT-PCR, using a nonhomologous
DNA competitor and an automated DNA sequencer.
Two major mechanisms are nonhomologous
end joining (NHEJ) and homologous recombination.
Endonucleases induce double-stranded breaks (DSB) in the DNA molecule that can later be repaired by the cellular DNA repair systems, via either blunt-end, nonhomologous
end-joining (NHEJ) or by homology-directed repair (HDR).
IgE cross-reactivity between the major peanut allergen Ara h 2 and the nonhomologous
allergens Ara h 1 and Ara h 3.