Tetrathionate / novobiocin
soup muller-kaufman (mkttn) (according to iso 6579)
Oka, "LC-MS/MS and centrifugal ultrafiltration method for the determination of novobiocin
in chicken, fish tissues, milk and human serum," Journal of Chromatography B, vol.
It is part of the routine of the laboratory to test the drugs most used by veterinarians, in this way the 17 active ingredients used in this sensitivity tests were: PMN = novobiocin
(40 [micro]g)+penicillin G procaine (40 [micro]g); CEQ = cefquinome (30 [micro]g); AMO = amoxicillin (10 [micro]g); DUL = danofloxacin (5 [micro]g); CTF = ceftiofur (30 [micro]g); CL = cephalexin (30 [micro]g); CN = gentamicin (10 [micro]g); TE = tetracycline (30 [micro]g); AM = ampicillin (10 [micro]g); ENO = enrofloxacin (5 [micro]g); NEO = neomycin (30 [micro]g); SUT = sulfatrim (25 [micro]g); AMC = ampicillin+colistin (30 [micro]g); CNM = cefalonium (30 [micro]g); ACA = amoxicillin (20 [micro]g) +clavulanic acid (10 [micro]g); OXA = oxacillin (1 [micro]g); and CIP = ciprofloxacin (5 [micro]g).
They found that all of the species isolated have sensitivity for enrofloxacin, fluorphenikol and ciprofloxacin; and also for amoxicillin, ampicillin, bacitracin, erythromycin, fucidic acid, gentamicin, chloramphenicol, lincomycin, nalidixic acid, neomycin, novobiocin
, oxytetracycline, cefoxitin and sulfamethoxazole-trimethoprim at varying levels.
discs were used to distinguish Staphylococcus saprophyticus, which is resistant to Novobiocin
in culture, from other coagulase-negative Staphylococci (CoNS).
5 % + Novobiocin
- PNP: p-Nitrophenyl; NaCl: sodium chloride; (+) positive test; (-) negative test.
Each 25 g sample of thawed ground beef was macerated in a stomacher (Bagmixer, Saint Nom, France) for 120 s with 225 mL of modified EC medium (Becton-Dickinson, Sparks, MD) with 8 mg/L novobiocin
solution (INLAB, Sao Paulo, Brazil) was added to the two broths.
5] cells/reaction were collected, washed with PBS and incubated with or without MDR inhibitors (verapamil for P-gp, NIK-571 for MRP1, and novobiocin
for BCRP) in the presence of the eFluxx-ID[R] Green probes for 30 min at 37[degrees]C and then evaluated by FACS analysis immediately.
and kaempherol were used as acceptor moleculesfor activity tests.
04 U), mannitol fermentation on Mannitol Salt Agar (MSA) (Merck, Darmstadt, Germany), urease activity, nitrate reduction, novobiocin
resistance, phosphatase, deoxyribonuclease (DNase) test and carbohydrate (xylose, sucrose, trehalose and maltose, fructose, lactose, mannose) fermentation tests .
1 mL of this sample was added to two broth cultures (Selenite cysteine and Tetrathionate broth) that contained iodine and novobiocin
, then was incubated at 35 [degrees]C and 41.