nutrient agar (grm-agar) 5 kg, indicated by the participants5.
All clinical pus samples after reaching the Microbiology department, were immediately inoculated in
Nutrient agar, MacConkey agar, and Blood agar and were incubated for 24 hours at 37 [degrees]C.
Sandy soil sample (1 g) was suspended in 100 mL of sterilized distilled water, slightly homogenized and spreaded onto
nutrient agar plates.
solani isolates were characterized as to their growth, color and sclerotia formation into three culture media,
Nutrient Agar (NA)(Himedia[R]), Potato Dextrose Agar (PDA) (Kasvi[R]) and Potato Sucrose Agar (PSA) (200 g potato, 20g sucrose, 20g agar, and 1000ml water).
Further the broth culture was inoculated on different selective and differential media like
Nutrient agar, Brain heart infusion agar, Mannitol salt agar, Mac Conkey Lactose agar and Eosine methylene blue agar and then incubated at 37 (o) C for 24 hours.
Microbial load was also determined inside and outside of fish as highest microbial load was observed in control group fish on both media
nutrient agar (NA) and eosin methylene blue (EMB) viz.
To obtain pure isolates, discrete colonies of pathogens isolated were subcultured by inoculating onto sterile
nutrient agar plates.
Isolates that grew at maximum values of pH were selected; distinct bacterial colonies on
nutrient agar plates were screened, purified by reculturing and stored at 4 AdegC on
nutrient agar plates and in 80% glycerol stock solution at -40 AdegC.
Enumeration of bacteria: All the collected effluent samples were serially diluted tenfold in sterile distilled water up to 10-5 dilutions and 0.1mL from each dilution inoculated in triplicate on pre-sterilized
nutrient agar plates.
A sterile pipette was used to extract 0.1 mL of the nutrient broth, which was poured onto a
nutrient agar plate.
For the preparation of
nutrient agar medium, 8 g of NB and 14 g of agar were dissolved in distilled water and final volume was made up to 1L.
All urine samples were cultured on
nutrient agar, blood agar, MacConkey agar and incubated at 37[degrees]C for 18-24 h.
nutrient agar - any culture medium that uses agar as the gelling agent