(grm-agar) 5 kg, indicated by the participants5.
All clinical pus samples after reaching the Microbiology department, were immediately inoculated in Nutrient agar
, MacConkey agar, and Blood agar and were incubated for 24 hours at 37 [degrees]C.
Sandy soil sample (1 g) was suspended in 100 mL of sterilized distilled water, slightly homogenized and spreaded onto nutrient agar
solani isolates were characterized as to their growth, color and sclerotia formation into three culture media, Nutrient Agar
(NA)(Himedia[R]), Potato Dextrose Agar (PDA) (Kasvi[R]) and Potato Sucrose Agar (PSA) (200 g potato, 20g sucrose, 20g agar, and 1000ml water).
Further the broth culture was inoculated on different selective and differential media like Nutrient agar
, Brain heart infusion agar, Mannitol salt agar, Mac Conkey Lactose agar and Eosine methylene blue agar and then incubated at 37 (o) C for 24 hours.
Microbial load was also determined inside and outside of fish as highest microbial load was observed in control group fish on both media nutrient agar
(NA) and eosin methylene blue (EMB) viz.
To obtain pure isolates, discrete colonies of pathogens isolated were subcultured by inoculating onto sterile nutrient agar
Isolates that grew at maximum values of pH were selected; distinct bacterial colonies on nutrient agar
plates were screened, purified by reculturing and stored at 4 AdegC on nutrient agar
plates and in 80% glycerol stock solution at -40 AdegC.
Enumeration of bacteria: All the collected effluent samples were serially diluted tenfold in sterile distilled water up to 10-5 dilutions and 0.1mL from each dilution inoculated in triplicate on pre-sterilized nutrient agar
A sterile pipette was used to extract 0.1 mL of the nutrient broth, which was poured onto a nutrient agar
For the preparation of nutrient agar
medium, 8 g of NB and 14 g of agar were dissolved in distilled water and final volume was made up to 1L.
All urine samples were cultured on nutrient agar
, blood agar, MacConkey agar and incubated at 37[degrees]C for 18-24 h.