One experiment was performed for each of the groups, and each was measured in octuplicate.
For the metabolic activity assay, the statistical analysis was performed by one-way ANOVA with Tukey's test on the means of octuplicate samples.
We used Cell Counting Kit-8 (Dojindo Molecular Technologies Inc., Japan) to perform cell proliferation analysis in octuplicate
according to the instructions of the manufacturer.
The reaction was carried out in octuplicate
by qPCR using the SmartChip Real-Time PCR System (WaferGen Biosystems) and TaqMan Fast Universal PCR Master Mix (2x) (Applied BioSystems, USA) according to manufacturer's instructions.
The results of cell growth activity were expressed as mean [+ or -] SD of experiments performed in octuplicate
. The effect on cell growth was expressed as a percent of the control and calculated as
Results (expressed as means [+ or -] SEM) derive from experiments performed in octuplicate
. Asterisks indicate significant differences as compared with the control group (cells maintained in the standard conditions).
After a shipping manifest is filled out in octuplicate
, it is hauled away by the waste disposal company.
All cellular measurements derive from three independent experiments, wherein each performed in octuplicates
, unless specified.