The optic lobe
is composed of two connected sections which are symmetrical, and in the center of it is optic lobe
The team focused on 59 "regions of interest" that showed especially high levels of calcium ion levels in the lobula, a part of the mosquito brain's optic lobe
. If the mosquito was shown a horizontal bar, two-thirds of those regions lit up, indicating increased neuronal firing in response to the visual stimulus.
Although spontaneous and evoked inhibitory synaptic potentials due to chloride fluxes have been recorded in the stellate ganglion of squids (Miledi, 1972) and in slices of the cuttlefish optic lobe
(Chrachri and Williamson, 2003), so far neither the neurotransmitter mediating those potentials nor their membrane receptors have been characterized.
For LM, samples including the optic lobe
and the optic stalk were immersed in Gilson fixative (2-6 h), and then well washed in running tap water, dehydrated and embedded in paraffin.
The structures that appeared on the dorsal aspect of the brain were the olfactory bulbs, cerebral hemispheres, optic lobe
, pineal body, cerebellum, and Medulla oblongata.
The squid optic lobe
contains 50%-80% of the neurons in the squid central nervous system and is therefore an excellent source of SVs for study of their biophysical and structural properties.
In the squid given two 36.8 mmol [l.sup.-1] doses of T-817 24-h apart (second group), HPLC analysis indicated a final optic lobe
concentration of 26.7 [micro]mo1 [l.sup.-1] (n = 10 squid).
The ACh release and Ch uptake were quantitatively studied through the use of in vitro chemiluminescence and isotopic methods on a subcellular fraction enriched in synaptic nerve endings (synaptosomes) isolated from cuttlefish optic lobe
. The ACh release evoked by [K.sup.+] depolarization was found to be very high (0.04 pmol ACh x [s.sup.-1] x [mg.sup.-1] protein).
For example, the three cortical layers of the cephalopod optic lobe
are organized similarly to the deeper layers in the vertebrate retina (Young, 1971).
These terminals contain conspicuous aggregates of polysomes and are responsible for essentially all the protein synthetic activity of optic lobe
Squid optic lobe
extracts were loaded onto a 5%-30% linear sucrose gradient and spun at 200,000 g for 14 h.
Nonspecific proteins were removed by several buffer washes; and a clarified squid optic lobe
extract was then passed over the column, followed by buffer washes.