Osmic achieved the biggest progress in the process of registering the military property of the Armed Forces of Bosnia and Herzegovina to the state of Bosnia and Herzegovina.
Furthermore, demyelination was a consistent histopathological feature in
osmic acid stained sections at the site of the lesion seven days after CSCI.
Cell suspensions were then rinsed twice with PBS and postfixed with 1%
osmic acid and 1.5% potassium hexacyanoferrate (II) solution (pH 7.3) and incubated at 4[degrees]C for 2h; then cell suspensions were dehydrated in a graded series of alcohol for 5 min each.
For transmission electron microscopy examination, 1 [mm.sup.3] renal cortex was fixed in 2.5% glutaraldehyde solution, rinsed by phosphate buffer, fixed in 10%
osmic acid, and then embedded in epon812 according to standard procedures.
Proximal colon tissues (n = 5) were fixed in 5% glutaraldehyde for 2 h at 4[degrees]C, postfixed in 1%
osmic acid (2h at 4[degrees]C), and stained with 2% uranyl acetate.
Two percent glutaraldehyde and 1%
osmic acid were added for fixation, and the plate was then embedded in epoxy resin 618, subjected to double staining with uranium and lead, and observed under a transmission electronic microscopy (Philips, CM10, 100 KV, Netherland) for the evaluation of ultrastructural changes.
As previously described, (25) the silica biofilm was fixed in 2.5% (v/v) glutaraldehyde in PBS (0.1M, pH 7.4) at 4[degrees]C for 2h, fixed again with 1%
osmic acid for 1 h, then rinsed with PBS, dehydrated through a series of ethanol dilutions, then treated with isoamyl acetate.
In Macedonia we opened our first call center in a very short time," said investor Mirsad
Osmic.
These tissues were immersed immediately into 2% glutaraldehyde in 0.1 M PB for 3 hours followed by 1%
osmic acid in 0.1 M PB for 2 hour at 4[degrees]C followed by dehydration with an ascending series of acetone, and then embedded in Epon (Nakarai, Kyoto, Japan).
She first referred to reduction of synovial inflammation with steroid injection and then talked about indication for its use, history,
osmic acid injections.
