When the generation of Acetyl-CoA exceeds the capacity of oxaloacetate
, the Acetyl-CoA is then diverted to the synthesis of ketone bodies, Figure IB, which include [beta]-hydroxybutyrate, acetoacetate, and acetone.
This fact occurs because there is no net production of oxaloacetate
to support gluconeogenesis since, in this case, fatty acids are catabolized to acetyl-CoA.
acetoacetate and oxaloacetate
, in the LC system and the ion source,
And the notion may be contributed by the acetyl-CoA and oxaloacetate
, the cleavage product of citrate required for the production of ROS .
At the end of the experimental period, the concentration of serum lipid profiles such as triglycerides (TG), total cholesterol (TC), High-density lipoprotein (HDL) levels were determined by enzymatic methods and using commercial kits and liver function test such as Serum glutamate oxaloacetate
transaminase (SGOT) and Serum glutamate pyruvate transaminase (SGPT) and Alkaline phosphatase (ALP) were done using Kits obtained from Span Diagnostics, India.
A partial list includes curcumin, artemisinin, silymarin, resveratrol, oxidative therapies, oxaloacetate
, methylglyoxal, methylene blue, Poly-MVA, and Argentyn silver.
Assessment was made on the basis of the development of tumours and their corresponding biochemical markers such as gamma-glutamyl transpeptidase, glutamate pyruvate transaminase, glutamate oxaloacetate
transaminase and alkaline phosphatase in the serum and in liver.
The analytical grade chemical reagents are pyruvic acid, oxalic acid, sodium citrate; oxaloacetate
, sodium bicarbonate, and FDP were purchased from Sigma-Aldrich, Inc.
Aspartate transaminase catalyzes the interconverssion of aspartate and a-Ketoglutarate to oxaloacetate
5 TLC = total leucocyte count; ESR = erythrocyte sedimentation rate; MCV = mean corpuscular volume; SGOT = serum glutamate oxaloacetate
transferase; SGPT = serum glutamate pyruvate transferase.
Significant change was not found in the plasma levels of Glutamate oxaloacetate
transaminase (GOT), glutamate pyruvate transaminase (GPT) and alkaline phosphate (ALP) or damage to liver tissue (Reitman and Frankel 1957; Wootton 1974; Nyblom et al.
CS activity was used as a matrix marker enzyme and determined by following the reduction of 5,5'-dithiobis(2-nitrobenzoic acid) at 412 nm coupled to the reduction of coenzyme A in the presence of oxaloacetate
(22) in a solution containing 0.