(a, b) Effect on liver function tests (LFT), serum glutamate pyruvate transferase (SGPT), and serum glutamate oxaloacetate
transferase (GOT) assay; (c) effect on MDA levels; (d) effect on antioxidant parameters.
(1) It is reduced to form lactate and NAD+; (2) it is oxidized by pyruvate dehydrogenase complex (PDHC) to yield acetyl-CoA; and (3) it is carboxylated by pyruvate carboxylase to form oxaloacetate
for the anaplerosis to replenish tricarboxylic acid (TCA) cycle intermediates.
Other Western meta-analysis has estimated its prevalence around 27%. In our study group, total 12 patients presented with raised serum glutamate oxaloacetate
transaminase (SGOT) and 11 patients had raised serum glutamic pyruvic transaminase (SGPT) with total 8 (14%) patients with elevation of both the transaminases with 15.7% in terms of SGOT and 10.5% for SGPT.
Patients were also excluded from the study if they were elderly (above 70 years), had a history of cerebrovascular disease, ischemic heart disease, were treated with beta-blockers or had chronic obstructive lung disease, psychiatric illness and/or active liver disease (defined as glutamine oxaloacetate
transaminase or glutamine pyruvate transaminase levels above 50 U [dL.sup.-1]).
Manganese homeostasis is of great importance to the organism because it is an essential mineral for the activity of several enzymes such as pyruvate carboxylase, which catalyzes the conversion of pyruvate to oxaloacetate
(16); arginase, which converts arginine to urea (17); and manganese superoxide dismutase (MnSOD), critical for antioxidant defense (18).
Phosphoenolpyruvate + [HC03.sup.-] [right arrow] oxaloacetate
 The reactions involved in the determination of AST activity are as follows, the oxaloacetate
produced by the transaminase serves as substrate for malate dehydrogenase by which it is reduced to malate in the presence of dihydronicotinamide-adenine dinucleotide (NADH), which is simultaneously oxidized.
The enzyme activity was measured in soleus muscle homogenates, and the amount of the complex resulting from acetyl-CoA and oxaloacetate
was determined at 412 nm and 25[degrees]C at an interval of 10 min.
Since aspartate is in fast equilibrium with oxaloacetate
, decreased AAT activity would lead to reduced labeling of oxaloacetate
M+4, which is reflected in citrate M+4.
Although both enzymes possessed malate dehydrogenase activity (converting oxaloacetate
to (S)-malate) , MJ1425 was identified as likely to be the biologically relevant ComC due to its kinetic parameters (higher [V.sub.max] and [V.sub.max]/[K.sub.M] for oxidation of (R)-sulfolactate by [NAD.sup.+] to sulfopyruvate) and was classified as a sulfolactate dehydrogenase (EC 220.127.116.117) [10, 11].
This effect can be implemented via using of AKG for synthesis of glycogenic amino acids such as glutamine [1,4] or via metabolizing AKG in Krebs cycle to oxaloacetate
, a well-known precursor in glucose biogenesis.
TSP, SG, SGOT, SGPT: Biochemical analysis of serum samples was done to estimate total serum protein by Modified Biuret method (Koller, 1984), Serum glucose by GOD-POD method (Tietz, 1976), serum glutamate oxaloacetate
transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) (Reitman and Frankel, 1957).