samples inactivation inactivation ([dagger]) ([dagger]) 7,3 +/+ ([double +/+ dagger]) 14, 3 -/- +/+ 21, 3 -/- +/+ * Infected skin tissues from 3 guinea pigs were fixed for 7, 14, or 21 d in 4% paraformaldehyde
, rinsed for 30 min in phosphate-buffered saline, and then homogenized.
The mononuclear cells were obtained from the pancreas and other tissues; the cells were procured in PBS, washed, subjected to red blood cell lysis, and suspended in PBS containing 1% paraformaldehyde
for analysis of fluorescence intensity using flow cytometry.
Cells were fixed with 4% paraformaldehyde
for 20 min, rendered permeable with 0.5% Triton X-100 (Sigma-Aldrich) for 20 min and blocked with rabbit serum (Boster Bioscience) for 30 min at room temperature.
In a three-necked round-bottomed flask equipped with a magnetic stirrer and a reflux condenser, paraformaldehyde
(1.8 g, 0.06 mol) and DMSO (20 mL) were taken and stirred at 50[degrees]C.
In breif, after pGH injections, the porcine hepatocytes were rapdliy isolated and fixed with 4% paraformaldehyde
for 20 min, then permeabilized for 5 min in 0.5% Triton X-100.
The pellets were then immunostained as follows: following fixation of the pellets with 4% paraformaldehyde
phosphate-buffered solution for 15 min at 4[degrees]C, cells were permeabilized with 0.1% Triton X-100, washed with PBS containing 2% BSA, and incubated with primary antibodies diluted in PBS containing 2% BSA.
After the endoscopy procedure, collected intestinal tissues were fixed with 4% paraformaldehyde
solution, embedded in paraffin block, and sectioned using a microtome.
It can take the form of formalin, a parapaste ingredient, or paraformaldehyde
. As a xenobiotic, it has impact on the immune system even at low concentration levels.
The right kidneys of fetuses were removed and fixed with 4% paraformaldehyde
and made into paraffin block.
Cells were preincubated for 10 min in Phosphate-Buffered Saline (PBS) and then fixed for 20 min with 4% paraformaldehyde
in PBS at room temperature.
Following incubation, the invaded cells attached to the lower surface of the membrane were fixed using 4% paraformaldehyde
and stained with 1% toluidine blue.
Cells seeded on sterile coverslips in 24-well plates were fixed in 4% paraformaldehyde
at 4[degrees]C for 30 min.