pararosaniline


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pararosaniline

(ˌpærərəʊˈzænɪˌliːn)
n
(Chemistry) a colourless crystalline alcohol, a component of the red dye fuchsin, also used as a biological stain
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After 5 days, biological material was fixed with 4% ice-cold paraformaldehyde and stained with pararosaniline. Colonies consisting of 50 or more cells were considered as colony forming units (CFU).
After 7 days, the cells were fixed with 4% ice-cold paraformaldehyde and stained with pararosaniline. Aggregates consisting of 50 or more cells were considered as colonies and visually scored as positive (Axio Observer A1 inverted microscope, Zeiss).
After electrophoresis, the gels were incubated overnight at 37[degrees]C in 3% sucrose, 0.5% Triton X-100, and 10 mg/mL dextran T10 in 10 mM sodium phosphate, pH 6.8, at 37[degrees]C, and the resulting glucan bands were treated with periodic acid and pararosaniline. The intensities of the stained bands were used to determine the activities of the GTF proteins.