Guo et al., "Inhibition of LSD1 by pargyline
inhibited process of EMT and delayed progression of prostate cancer in vivo," Biochemical and Biophysical Research Communications, vol.
Specific activity was determined in the presence of pargyline
Homogenates were distributed in 96-well dark microplates (at 50 [+ or -] 5 [micro]g protein/well) and incubated at 37[degrees] C in the dark for at least 30 min in 200 [micro]L final volume after a 30 min preincubation without (control) or with 1 mM pargyline
or semicarbazide to inhibit MAO or SSAO activity, respectively, or even with both reference inhibitors to abolish all AO activity.
1,3-Ditolylguanidine (DTG), clozapine, GBR12909, pargyline
and dopamine were purchased from Sigma Chemical Co.
Central serotonin level dependent changes in body temperature following administration of tryptophan to pargyline
and harmaline- pre treated rats.
Exogenous analogues of these compounds are antidepressants such as pargyline
, deprenyl, and so on.
prevents MPTP-induced parkinsonism in primates.
Addition of the monoamine oxidase inhibitor pargyline
together with BH4 also did not increase serotonin levels (n = 3, Fig.
Certain medications (phenelzine, pargyline
, corticosteroids) can also precipitate similar symptoms, as can a host, of disorders of the hepatic, renal, hematologic, and endocrine systems, and malignancies.
Isoform-specific irreversible inhibitors include clorygline, which is a research compound specific for MAO-A, and the two B-selective inhibitors selegiline and pargyline
. Reversible MAO inhibitors include moclobemide, brofaromine, toloxatone, and befloxatone, all of which are specific for MAO-A.
Three studies used a monoamine oxidase inhibitor (deprenyl and pargyline
[Eutonyl]), and the others tested more commonly used antidepressants, including desipramine (Norpramin), bupropion (Wellbutrin), venlafaxine (Effexor), fluoxetine (Prozac), and sertraline (Zoloft).
In particular, 40 [micro]L of tissue homogenates was preincubated in 100 [micro]L of PBS, pH 7.8, at 37[degrees]C for 30 min in the absence or in the presence of the MAO inhibitors pargyline
(100 [micro]M; Sigma-Aldrich, St.