This fact is evident in the pereiopod
muscles of the diverse crustacean species (Neil et al.; Gruhn & Rathmayer; Longo et al.; Longo & Diaz 2013a,b).
Structural properties of bimodal chemo- and mechanosensitive setae on the pereiopod
chelae of the crayfish Austropotamobius torrentium.
Hemolymph samples were drawn from individual animals at the base of the fifth pereiopod
. Crabs were anesthetized on ice and the sixth and seventh gill pairs (G6 and G7) were excised.
Approximately 200-300 [micro]L of haemolymph were extracted from the arthrodial membrane of the fifth pereiopod
of each organism; using a 1 mL syringe rinsed with a 10% cooled anticoagulant solution of sodium citrate.
The gonad, hepatopancreas and the muscle of the second right-hand pereiopod
of each specimen were extracted and held for 48 h at 60[degrees]C to obtain the dry weight (Corgos & Freire, in press).
To determine hemolymph osmolality, samples were collected from the base of the fifth pereiopod
In some experimental animals, a high-frequency resonance in the transducer was generated by the pereiopod
A small section (approximately 2.5 mm piece) of adult pereiopod
soft tissue and the entire lobster larva were processed for genomic DNA.
The antennules (first antennae), the pereiopod
dactyls, and the mouthparts are the primary chemoreceptor organs (Ache, 1982).
In the laboratory, a segment was cut from each pereiopod
, and the inner soft tissue removed and processed for the isolation of genomic DNA as described (Crivello et al.
Members of clade A showed the greatest mean divergence (0.0108); taken as a natural group, they might be characterized morphologically by the presence of an epipod on third maxilliped, pleurobranchiae on fifth pereiopod
, ventral and dorsal rostral teeth, as well as by the absence of branchiostegal, pterygostomian and parapenaeid spines, and transverse and longitudinal sutures on carapace.
Stage IVB/C larvae, found only among animals ablated at [t.sup.30] (Table 2), exhibited most of the characteristics of Knowlton's stage IVC, but only the exopod of pereiopod
4 showed reduction in size and loss of swimming function.