A pint of citrated blood was added to the perfusate
after 50 min.
24 hours while measuring pressure, temperature, flow rate of the perfusate
and the flow resistance of the graft to the ultrasonic detection of air bubbles, and the possibility of collection of perfusate
for laboratory purposes, including subsequent supply of expendable supplies according to the specifications in the tender documents lifetime instrumentation equipment, i.
In the I/R group, the Krebs–Henseleit solution was add to perfusate
at the onset of reperfusion.
3], pH, and NO metabolites of the perfusate
were measured during steady-state and at the end of experiments (30 minutes).
Treatment of bladder tumors and benign prostatic hyperplasia with a new TUR system using physiological saline as perfusate
double dagger]) Preparation of kidneys for transplantation included an in situ flush (initiated 25 min after the donor was declared brain dead and was extubated) with 2 L of University of Wisconsin solution (15), each of which was infused in [less than or equal to]4 min; a flush with 200 mL of this solution after the kidneys were explanted; and continuous circulation with kidney perfusate
solution until the kidneys were transplanted.
Nevertheless, the measured inflow at 100 mmHg resulted in a perfusate
of 100 [micro]l/(min [cm.
Once the cannulation of left atria was complete, aortic inflow line was closed, the atrial inflow line to perfuse the heart in non-recirculating working heart mode at the rate of 23-25mL/m , such that the perfusate
entered the left atria through pulmonary vein, passed through left ventricle and was pumped out through aorta.
Vessels were stimulated with SFH (1 [micro]g/ml or 3 [micro]g/ml) and 150 [micro]l of perfusate
taken 5 min later.
Coronary flow (CF) was measured by the timed collection of the perfusate
dripping from the heart into a graduated cylinder.
was maintained at 37[degrees]C by a circulating water bath and gassed continuously with 5% C[O.
samples were analyzed for glutamate content by high-performance liquid chromatography (HPLC) (column, CAPCELL PAK C18 UG120A [1 mm X 150 mm]; Shiseido Co.