Ascorbate-[Fe.sup.2+] Lipid Peroxidation
of Rat Liver Microsomes: Effect of Vitamin E and Cytosolic Proteins.
Oxidative stress and reactive oxygen species like superoxide anion and hydrogen peroxide, decrease sperm motility as well as membrane integrity due to increase in membrane lipid peroxidation
High amounts of polyunsaturated fatty acid are found in the mammalian spermatozoa membranes, thereby making them susceptible to lipid peroxidation
. Although, free radicals and ROS play major roles in reproduction, they are strongly associated with oxidative stress.
isoxidative damage of polyunsaturated fatty acids mediated by free radicals.
In the human body, high levels of constantly formed free radicals and other ROS such as superoxide anion ([O.sub.2] [.sup..]-), hydrogen peroxide ([H.sub.2][O.sub.2]), and hydroxyl radicals ([.sup..]OH) are involved in the generation of cascade reactions that attack cell membrane phospholipids and induce membrane lipid peroxidation
. Accumulation of damaged macromolecules is a major contributor of aging and other degenerative diseases.
In the body, reactive oxygen species cause various types of damage like lipid peroxidation
and cellular damage.
To demonstrate lipid peroxidation
in mice liver, extract - sodium nitroprusside (5uM) or ferrous sulphate (10uM) was introduced to produce thiobarbituric acid reactive species (TBARS).
The researchers tracked a number of indicators of aging, including glutathione (GSH) content in the brain and liver, lipid peroxidation
, and protein responses.
occurs at the cell membrane by the effect of free radicals.
has been implicated in the etiology of several diseases.
Measurement of Lipid Peroxidation
Levels in the Liver.
In conclusion, it was supposed that elevation in serum ADA activity was due to cell mediated immune system stimulation by Mycobacterium avium subsp and the increase in MDA level might be due to the increase in lipid peroxidation
because of affected membrane lipids by infection.