isolation by detergent free method was used to study role of Lyn in phagocytosis.
Blander JM, Medzhitov R (2004) Regulation of phagosome
maturation by signals from toll-like receptors.
Each phagocytic event results in the formation of a phagosome
into which reactive oxygen species and hydrolytic enzymes are secreted.
Modeling the reactions of superoxide and myeloperoxidase in the neutrophil phagosome
. J Biol Chem 2006; 281:39860-869.
We're looking at the phagosome
, which engulfs the bacteria, and looking to see if there are specific proteins in the phagosome
that are induced upon acquiring Brucella.
The paper by Gagnon et al., titled 'Endoplasmic Reticulummediated Phagocytosis Defines a Novel Mechanism of Entry in Macrophages,' used Caprion's CellCarta subcellular proteomic approach to generate a comprehensive description of the proteins which make up the phagosome
, the organelle responsible for destroying intracellular pathogens.
Natural resistance to infection with intracellular pathogens: the Nrampl protein is recruited to the membrane of the phagosome
. J Exp Med 1997; 185:717-30.
Lerner speculates that antibodies produce an extra dollop of hydrogen peroxide within the phagosome
by converting this singlet oxygen into superoxide anions, just as antibodies do in the experiments where the singlet oxygen is generated by ultraviolet light.
The sequential process including cup formation, phagosome
internalization, and phagolysosome formation is critical steps in the process of phagocytosis .
pneumophila in macrophages, for avoidance of phagosome
acifidication and lysosome fusion and is necessary for instruction of apoptosis in human macrophages (21-23).
Kettle, "Modeling the reactions of superoxide and myeloperoxidase in the neutrophil phagosome
," Journal of Biological Chemistry, vol.
PBDs-treated cells showed a double-membrane phagosome
within some of the destructed organelles as shown in Figure 4(b)(C) while Figure 4(b)(B) demonstrated condensation and fragmentation of the nucleus accompanied by a decrease in cytoplasm to nucleus ratio.