For PPRE-H2B-eGFP experiments, cells were incubated with Alexa Fluor[R] 555 Phalloidin
(#A34055, Invitrogen; 1:200 in PBST) for 1 h, in the dark at room temperature, then washed three times in PBST, and counterstained with DAPI for 10 min at room temperature, as described in Degrelle et al.
CD169+ cells are shown in red in both samples, while the light blue staining indicates the white pulp in the mouse spleen (combination of anti-B220 and anti-CD3 antibodies), and the follicles and endothelium in the pig spleen (phalloidin
Confocal images of thoracopods stained with phalloidin
to mark actin show striated muscle fibers running from the basis to the proximal edge of the distalmost segment of each endopod and exopod (Fig.
Following the incubation period, cells were double stained with phalloidin
and acridine orange at room temperature.
At 6 h (Figure 4B), the area of actin fibers stained with phalloidin
was higher and after 24 h of adhesion, a spread morphology can be observed (Figure 4C).
To visualize actin filaments, after the secondary antibody incubation, the zygotes were treated with a 200-fold dilution of rhodamine phalloidin
(R415; Molecular Probes) by DW for 2 h at room temperature and then rinsed in rinse solution three times.
In order to detect the area of cells, the attached cells were fixed in 4% paraformaldehyde (Sigma, USA) for 15 minutes and dyed by phalloidin
for 1 h, and fluorescence was measured and analyzed by Image J.
Caption: Figure 5 (a) Confocal microscope images of rhodamine phalloidin
and DAPI stained adipose derived stem cells (ADSCs) cultured on POSS-PCL and POSS-PCU surfaces for 24 hours after different sterilisation techniques (b) Te circularity quantification of the cultured adipose derived stem cells (ADSCs) seeded on POSS-PCL and POSS-PCU surfaces for 24 hours after different sterilisation techniques
After 60 min, the cells were fixed with 4% paraformaldehyde and stained with DAPI and fluorescein isothiocyanate- (FITC-) conjugated phalloidin
. The images were captured using a Nikon eclipse Ti.
Filamentous actin (F-actin) was labelled with 20 [micro]g/mL phalloidin
conjugated with fluorescein isothiocyanate (FITC; Sigma-Aldrich, Germany) for 1 h at room temperature.
After washing with PBS, rhodamine fluorescence phalloidin
(1 : 200, Sigma, St.