phosphate buffer solution


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Noun1.phosphate buffer solution - a solution containing a phosphate buffer
buffer solution - a solution containing a buffer
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The electrochemical detection was made through pulse differential voltammetry using phosphate buffer solution (pH 7.4) as electrolyte.
0.1 M phosphate buffer solutions (PBS) with various pH values were prepared by dissolving an appropriate amount of [Na.sub.2]HP[O.sub.4] in water and adjusting the pH values with 0.1 M HCl or NaOH solutions.
Samples were diluted with 10 mM phosphate pH 7.0 for native conditions and with 10 mM phosphate and 5 M GdnHCl buffer solution pH 7.0 for denaturing conditions, while for denaturing-reducing conditions samples were treated with 10 mM DTT for 30 min at 25[degrees]C and buffer exchanged using NAP-5 columns against 10 mM phosphate buffer solution and GdnHCl 0.5 M pH 3.0.
0.5 g sodium carboxymethylcellulose in 100 ml 0.2 M phosphate buffer solution) for carboxymethyl cellulase (CMCase) activity, from 0.5% avicel microcrystalline cellulose (i.e.
When the signal of the oxygen electrode stabilized in the 50 ml phosphate buffer solution, the certain amounts of calibration solution were added to achieve known [BOD.sub.7] values to the measurement solution.
Addition of 10 [micro]L of urine to 50 [micro]L of TMB (tetramethylbenzidine) solution, followed by addition of 500 [micro]L of 0.1 M phosphate buffer solution, caused a dramatic color change of the specimen to dark brown.
A phosphate buffer solution at pH 6.3 and a commercially available Pilsen beer, with pH 4.2, 2.5 Brix and 4.5 GL alcohol content, were inoculated with a suspension of L.
The in-vitro studies were carried out soaking the specimens in sodium phosphate buffer solution as the medium for release of drug at pH 7.4 and 37[degrees]C.
All kinetic measurements were carried out under continuous stirring in an airtight thermostatted glass cell at 25[degrees]C in air-saturated 0.1 M phosphate buffer solution (pH 7.0) [5, 19].
In additional test series, this control sample was supplemented with 100 [micro]L volumes of a phosphate buffer solution containing 8 and 10 nmol of Trp (final concentrations, 62.2 and 72.2 [micro]mol/L) or 0.8 and 1.0 nmol of Kyn (final concentrations, 4.81 and 5.81 [micro]mol/L).
Test solutions consisted of (i) distilled water, (ii) pH 3.5 citrate phosphate buffer solution, (iii) citrate phosphate buffered 0.001 M G[A.sub.3] solution, (iv) citrate phosphate buffered 0.005 M G[A.sub.3] solution, and (v) citrate phosphate buffered 0.01M G[A.sub.3] solution.
1M Phosphate buffer solution (pH 7.4) 100 mL Distilled water 800 mL Combine the ingredients.