Pretreatment with SB203580 reduced the expression of phospho-
p38 MAPK, and pretreatment with SC-514 reduced the expression of phospho-p65 protein.
For the sake of bar-graph clarity (b), SMEM values of all experiments were normalized to 1 in order to represent mean ratios of phospho-
ACC/total protein content and phospho-
AMPK/AMPKt in different experimental conditions (b).
The antibodies used were against Cathepsin K, TRAF-6, IRAK-1, TAK-1, NIK, IKK[alpha], IKK[beta], phospho-IKK [alpha]/[beta], NF-[kappa]B p65, Phospho-
NF-[kappa]B p65, I[kappa]B[alpha], Phospo- I[kappa]B[alpha], and [beta] actin (Santa Cruz Biotechnology Inc.
unsaturated, omega-3, 6, and 9 fatty acids, phospho-
and glycolipids, cholesterol and other sterols, and lipophilic vitamins.