The nucleotide identities of predicted MojV genes exhibited similarity with genes of known henipaviruses: N (53.0%-57.0% identity), phosphoprotein
(37.8%-43.0% identity), matrix (59.5%-63.4% identity), fusion (47.5%-51.4% identity), attachment glycoprotein (36.6%-41.8% identity), and L (55.9%-58.6% identity) genes.
Assays designed to detect changes in expression of RNA, proteins (both secreted and intracellular), and phosphoprotein
targets are now commonly used to analyze blood samples.
KF958252) consists of 11,141 nt and has a typical rhabdovirus gene arrangement of 5 major open reading frames (ORFs) in the order 3'-nucleoprotein (N), phosphoprotein
(P), matrix (M) protein, glycoprotein (G), and large (L) protein-5' (Figure, panel A, Appendix, wwwnc.cdc.gov/ EID/article/20/6/13-1880-F1.htm).
Unfortunately, we were unable to include flow cytometric analysis of vasodilator-stimulated phosphoprotein
Amplified products ([approximately equal to] 238 bp from the nucleoprotein [N] gene and [approximately equal to] 425 bp from the phosphoprotein
[P] gene) were sequenced and compared with sequences of DMV and other morbilliviruses by using BLASTN (http://blast.ncbi.nlm.nih.gov/Blast.cgi).
Examples of these upregulated genes include LALBA  (lactalbumin, alpha-), which encodes a principal milk protein that enables lactose production; CCKAR (cholecystokinin A receptor), which encodes a major physiological mediator of pancreatic enzyme secretion and smooth muscle contraction of the gallbladder and stomach; HCRTR2 [hypocretin (orexin) receptor 2], which encodes a protein involved in stimulation of food intake; MCHR1 (melanin-concentrating hormone receptor 1), which encodes a protein involved in neuronal regulation of food consumption; and DMP1 (dentin matrix acidic phosphoprotein
1), which encodes an extracellular matrix protein crucial for proper mineralization of bone and dentin (see Table 2 in the online Data Supplement).
ORF1 encodes a nonstructural protein of 1,596; ORF2 encodes a capsid protein of 654 aa, and ORF3 encodes a functionally unknown phosphoprotein
of 108 aa.
A relevant target of both cyclic nucleotide-regulated protein kinases is the focal adhesion protein vasodilator-stimulated phosphoprotein
(VASP) (22, 23), which is strategically involved in platelet inhibitory pathways.
We extracted RNA from frozen lung samples by using TRIzol Reagent (Life Technologies Corporation, Carlsbad, CA, USA) according to the manufacturer's instructions and amplified a 374-bp conserved fragment of the phosphoprotein
(P) gene by reverse transcription PCR.
A candidate gene for PXE susceptibility is SPP1 (secreted phosphoprotein
1; previously OPN, osteopontin).
Although this case occurred nearly 9 years ago, the nucleotide sequence of a fragment of the morbillivirus phosphoprotein
(P) gene shows high homology with sequences recently reported for morbilliviruses from striped dolphins (Stenella coeruleoalba) in the Mediterranean Sea.
The proteins in Table 1 that do not derive from these previously discussed categories include cytoskeletal proteins (actin [beta], CAPZ [beta] subunit, cofilin, and stathmin 1), transport-related proteins (calbindin 1, fatty acid binding protein 5, transthyretin, and tetranectin), proteolytic proteins (cathepsin D and proteasome subunit [alpha] type 5), and proteins with other or unknown functions (stress-induced phosphoprotein
1, peptidylprolyl isomerase A, oncogene DJ1, TPA:LRRC15, neuropolypeptide h3, esterase D, pyrophosphate phosphorhydrolase, carbonic anhydrase, and calmodulin 3).