PBMC proteins were extracted in 60 [micro]L phosphorolysis
buffer containing 100 mM NaCl, 0.1% SDS, 1% Nonidet P40, 50 mM Tris, pH 7.4, 10 mM EDTA, 10 mM p-nitrophenolphosphate, 40 mM b-glycerophosphate, and Complete Protease Inhibitor Cocktail Tablets (Roche, Mannheim, Germany).
It catalyzes the first step of glycogenolysis, in which glycogen is converted to glucose 1-phosphate by phosphorolysis
in the presence of inorganic phosphate, and through mobilization of glycogen, it is primarily associated with provision of an emergency glucose supply during periods of hypoxia and hypoglycemia.
The products from hydrolysis and/or phosphorolysis
of starch are subsequently metabolized through the glycolytic and oxidative pentose phosphate pathways to give products that are either metabolized by the chloroplasts or exported to the cytoplasm for sucrose biosynthesis (Stitt, 1990).