Pieces of kidney of about 3mm3 were cut with sharp cutter and immediately shifted to Bouin's fixative (combination of 70 ml of supernatant of water saturated Picric acid
, 25 ml of formalin and 5ml of glacial acetate) for fixation for 48 hours and subsequently preserved in ethanol (70%).
Arayne, Synthesis and Spectrophotometric Study of Omeprazole Charge Transfer Complexes with Bromothymol Blue, Methyl Orange, and Picric Acid
, 9, 21749 (2015).
For histological analysis, samples were fixed in paraformaldehyde (diluted at a 1:9 ratio with 96% ethanol), and for immunohistochemistry, in Bouin's solution (71% saturated picric acid
, 24% formaldehyde, 5% acetic acid).
Serum was stored at -20[degrees]C for future analysis of alanine aminotransferase (ALT; Reitman-Frankel colorimetric method), aspartate aminotransferase (AST; Reitman-Frankel colorimetric method), [gamma]-glutamyl transferase (GGT; Szasz method), urea N (urease method), creatinine (picric acid
colorimetric method), and uric acid (phosphotungstic acid colorimetric method), using an automated chemistry analyzer (Hitachi 7080, Beijing CIC Clinical Laboratory, Beijing, China).
The munitions freighter, built in England in 1899 and purchased by the French shipping firm Compagnie Generate Transatlantique, carried 2,300 tons of wet and dry picric acid
, 200 tons of TNT, 10 tons of gun cotton, and 35 tons of benzol.
Fang, "A novel picric acid
film sensor via combination of the surface enrichment effect of chitosan films and the aggregation-induced emission effect of siloles," Journal of Materials Chemistry, vol.
However, if [H.sub.2]O were the preferred decomposition product, the specific enthalpy of decomposition drops to just less than that of picric acid
, the lowest-energy HEDM in the table.
Only right ovary was evaluated to maintain uniformity and prevent any discrepancy as suggested by the literature.11 Afterwards, the ovaries were preserved in Bouin's fluid for 12 hours followed by immersion in 70% ethanol saturated with lithium carbonate, to remove excessive picric acid
. After processing, 5um thick serial sections were prepared using rotary microtome.
In a May 19, 2009 email sent from then deputy chief and current fire department chief Kevin McCarthy, the New Jersey State Police's bomb squad removed half a pound of picric acid
, an explosive likened to TNT, from the Heller site.
Then the sections were stained with picric acid-acetone mixture (5 mL saturated picric acid
and 45 mL acetone) for 30 s and decolorized 3 times with acetone.
In the next step the specimens were etched with nital 5% and electrolytically polished in a solution of picric acid
(100 ml ethanol, 18 ml H2O, 6 ml glacial acetic acid, 12 g picric acid
98%) for 7s.
Other reagents such as bromine phenol blue, xylene, ethanol, formaldehyde, acetic acid, picric acid
, orchid agent, bleaching agent, hematoxylin, eosin and ultra pure water were domestic and analytical pure.