to jointly develop and operate a 63,000- square foot Cannabis Biotech Complex, to be the first of its kind in Western Canada to house a DNA Botany lab, CBD extraction facility and Tissue Culture Plantlet
Production facility to service the Cannabis market and agriculture market in general
Thus, the production of pre-sprouted plantlets
(PSP) is an alternative technology to conventional planting that allows greater control over plantlet
quality, vigor and health (Landell et al., 2013).
Keeping in view the importance of above discussed prospecitve, the present study was conducted to study the impact of thermotherapy duration on potato plantlet
survial and virus elimination efficiency in tested potato cultivars.
The same herbicides however, if applied after planting, may affect budding and plantlet
development (REINHARDT and CUNHA, 1993).
Media Preparation for Callus Induction, Callus Regeneration, and Plantlet
The LICC is a model facility for farmer training, demonstrations and trials, plantlet
production, and sludge composting, with a station for buying coffee beans from farmers.
This genotype had the best results in the number of regenerated plantlets
per bud (0.15 plantlet
), though there was no significant difference between Cadia, Magno, and Maratus.
The appropriate medium formulation for adventitious shoot induction and proliferation of sterilized red pitaya explants was Murashige and Skoog's medium (MS) supplemented with 5.5 mg/L 6-benzyladenine (6- BA) and 0.1mg/L a-naphthaleneacetic acid (NAA), and maximum average number (6.4) of adventitious shoots per explant and maximum average plantlet
height (2.8 cm) were achieved within 30 days.
Therefore, there is a need for such a protocol that does not require plantlet
regeneration through somatic embryogenesis and the best option to this is direct transformation of commercial germplasm through shoot t ip/ apices culture.
The experimental unity consisted of one test tube containing a single plantlet
. Data were processed by ANOVA and the mean differences were compared by Scott-Knott test, using the software Assistat ver.
Specifically, the effects of BAP, NAA and [GA.sub.3] used either alone or in combination were evaluated for their effect on plantlet
regenerative capacity, multiple plantlet
production, shoot height, average leaf number per shoot and average node number per shoot at ten weeks after meristem culture.