In addition, the amounts of both PEI and plasmid DNA
were reduced up to 66[degrees]o and 2500, respectively compared to our previous protocol.
of pND vector was extracted to be used in sub-cloning experiment.
Pellets were resuspended in 0.9% sterile saline, and the final concentration of plasmid DNA
in the chitosan-DNA complex was 2.5 mg/mL.
DNA templates prepared by modified plasmid preparation method showed high yield and relative less purity compared to the templates purified through MINI-PREP 96[TM], an automated plasmid DNA
preparation instrument (Figure 1) and QIAprep (gel picture not shown).
Analysis of biomarkers after intramuscular injection of Vaxfectin(R)-formulated hCMV gB plasmid DNA
. Vaccine 2009; 27(52):7409-7417.
A single reaction mixture contained 1[micro]l of plasmid DNA
extract for Enterobacter species, E.coli and Klebsiella species, 100pmol of each primer, 100 [micro]M (each) dNTPs, 1.25 U Taq polymerase (Taq DNA polymerase kit-201203, Quiagen, Germany) and buffer with 1.5 mM Mg[Cl.sub.2] supplied along with the kit in a total volume of 50 [micro]l.
obtain plasmid DNA
, such as pBr322, to use for the activity and scrutinize a restriction enzyme map of the DNA.
With respect to the material shipped to the participants in EQAP1, we provided 2 samples, a purified PCR-product and plasmid DNA
. Specifically, sample A was a dried pellet of a 310-bp PCR product (160 ng), and sample B was a plasmid DNA
preparation (2 /[micro]g) with an insert of 2355 bp.
The first contract, awarded in April 2000, focused on adjuvant-enhanced plasmid DNA
(pDNA) and peptide-based vaccines.
Endonucleolytic activity from Micrococcus luteus that acts on gamma ray induced damage in plasmid DNA
Headquartered in Seattle, Washington, the company's technology platform targets solid tumors based on transcriptional activity using a unique lipid nanoparticle and plasmid DNA