The shrinking, collapse and depression in the cells could be attributed to ex-osmosis of cytoplasmic contents that resulted in plasmolysis
of bacterial cells or imperfect synthesis of the cell wall after treatment.
High salinity can result in the loss of microbial activity and cell plasmolysis
. However, moderately salt-tolerant bacteria can resist or reduce the damaging effects of salt concentrations of up to 5-20% salinity .
It can be attributed to the fact that short-time action of high-temperature blanching generally produces profound changes to the cell microstructure including protoplasm coagulation, water loss and shrinkage of intercellular spaces, plasmolysis
, increase in permeability or even disruption of cell membranes, and decrease in bound or hydrophilic capacity of extracellular and intracellular water [29-31], which definitely contributed to faster water-evaporating speed and higher mass loss in HWB-VF-FD.
Kiehl (2008) reports that such conditions lead to dehydration due to cellular plasmolysis
, making it difficult to establish earthworms.
As a result it will cause plasmolysis
which is the process of shrinkage of the cell as a result of loss of water from the protoplasm to the environment.
vulgaris when treated with 40-50 mg [L.sup.-1] during 72-120 h of exposure, and this inhibitory effect was caused by cellular morphological alterations such as plasmolysis
(leak of cytosol), cytomembrane breakage (detached or degraded plasma membrane), and disorder of thylakoids (grana lamella).
The amount of sugar added likely caused high osmotic pressure in the cells, resulting in plasmolysis
and decreasing both the fermentation rate and sugar utilization.
cellular level brought about by plasmolysis
and deplasmolysis of epithem
In the present study it is proposed that the extracts caused membrane damage during the one hour of incubation by increasing the membrane permeability and this caused the decrease in absorbance since plasmolysis
did not occur when the cells were placed in PBS containing NaCl.
As the salt concentration increased further (hypertonic), the process was reversed which resulted in further plasmolysis
of the green pepper cells, thereby drastically reducing its moisture content, hence, reduced drained weight.
As a result, the cell structure and cell wall in some areas were destroyed, and plasmolysis
began to occur (Figure 6(a)).