Although tRNA plays a crucial role in RNA maturation from polycistronic
transcripts, many bivalve mitogenomes deviate from the standard 22 tRNA.
lentiviral vector for "hit and run" reprogramming of adult skin fibroblasts to induced pluripotent stem cells.
The Yamanaka factors-inducible embryonic stem cell (ESC) possesses the optimized reverse tetracycline-dependent transactivator (MS- rtTA) at the Rosa 26 locus and a polycistronic
cassette encoding the four reprogramming factors (Oct3/4, Sox2, Klf4, and c-Myc), followed by ires-mCherry at the Col1a1 gene locus under the tetracycline-dependent promoter (tetOP).
micro- RNA cluster, miR-17-92, is over-expressed in human lung cancers and enhances cell proliferation.
Tatematsu et al., "A polycistronic
microRNA cluster, miR-17-92, is overexpressed in human lung cancers and enhances cell proliferation," Cancer Research, vol.
Al-Bassam et al., "Polycistronic
RNA polymerase II expression vectors for RNA interference based on BIC/miR-155," Nucleic Acids Research, vol.
It was shown then that both inheritance and polymorphism are determined by polycistronic
genetic loci located on the long arms of wheat chromosomes 1A, 1B, and 1D [1, 2].
Several studies have improved the in vivo reprogramming efficiency even further by optimizing polycistronic
expression vectors to control the stoichiometry of TF expression or by the addition of small molecules delivered with TFs [27, 55-57].
Biogenesis of miRNA genes initiates with their transcription by RNA polymerases II, which gives rise to monocistronic or polycistronic
long primary transcripts named primary miRNA or pri-miRNA, with a length of 200 nt to several kilobases, folded into hairpin structures containing imperfectly base-paired stems.
The miR-17-92 cluster as a polycistronic
gene located in human chromosome 13 ORF 25 (c13orf25) is composed of 7 mature miRNAs .