polylysine


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polylysine

(ˌpɒlɪˈlaɪsiːn)
n
(General Physics) microscopy a type of synthetic polypeptide used to enable adhesion of cells to microscope slides
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Histological sections were loaded onto polylysine slides, de-paraffinized in xylene, rehydrated in graded ethanol dilutions and distilled [H.sub.2]O, and then stained with hematoxylin and eosin (HE).
Professor Ken Yung said, "The novel matrix enables scientists to cultivate NSCs by adopting the usual method, however with the added advantage of organic compounds (like Polylysine and Polyornithine) being excluded from the process, thereby reducing the potential risk of carcinogenesis or inflammation in stem cell therapy.
For immunohistochemistry, the tissue sections were mounted on the slides coated with polylysine. The sections were stained with monoclonal antibody for CD8+ (anti-rat CD8+, Novocastra, RTU-CD8-295, Cat.
Polylysine linkers bearing several ce6 molecules were covalently attached in a site-specific manner to partially reduced IgG molecules, which allowed photoimmunoconjugates to bear either cationic or anionic charges.
In this study, cationic polypeptide polylysine (polylys) was fused with SM5-1 scFv (SMFv) which was derived from SM5-1 mAb.
For immunohistochemistry, thin sections of spleen and BF were mounted on the glass slide coated with polylysine. The slides were stained with monoclonal antibody for CD4+ (anti-rat CD4+, Novocastra, RTU-CD4-1F6, Cat.
The glasses were previously left in solution of polylysine at a concentration of 1 mg/ml in a Petri dish for 12 hours, then the glasses were air-dried for 2 hours.
The first widely applied strategy was to combine the commercial iron oxide nanoparticles (e.g., Feridex[R] or Resovist[R]) with the commercial transfection agents (e.g., liposomes, polylysine, or protamine sulfate) for the efficient labeling, but such shortcomings as imprecise surface properties and noncompletely consistent sizes of combinations influenced the results of tracing and imaging [9, 10].
Likewise, polylysine and try-paflavine inhibit miRNA-mediated gene regulation and thereby attenuate tumorigenic conditions.
The next step was the formation of a semipermeable membrane on the surface of the gel beads induced by addition of polylysine (Sigma Chemical Co., St.
High efficiency transfer of cystic fibrosis transmembrane conductance regulator cDNA into cystic fibrosis airway cells in culture using lactosylated polylysine as a vector.
The only difference was glass slides used for immunohistochemistry that were coated with 0.1% polylysine. After placing the slides in incubator for 24 hr, paraffin was removed with xylene and it was taken with descending grades of alcohol, tissue sections for antigen retrieval, respectively in 10 mM citrate buffer with pH=6, were put for 20 min at temperature 90 [degrees]C.