Considering all the aspects of producing and selecting aptamers specific to targets, a DNA polymerase
can be regarded as the most important factor that determines the success of creation of a library of variable ssDNA aptamers.
0 s/cycle (hence the name extreme PCR), and then increased the rates of primer annealing and polymerase
extension by increasing primer and polymerase
concentrations to match the cycling speed.
Keywords: Influenza A virus PB1-F2 polymerase
activity minigenome system.
In addition to DNA ligase, the reaction mix needs also to contain DNA polymerase
and only the "missing" dNTPs.
The product of the gene, Taq DNA polymerase
was also isolated.
We know that polymerases
and clamp loaders can't bind the sliding clamp at the same time, so the hypothesis was that clamp loaders latched sliding clamps onto DNA, then left for some time during DNA replication, returning only to unlatch the clamps after the polymerase
left so they could be recycled for further use," he added.
ABBREVIATIONS: bDNA = Branched DNA; DNA = Deoxyribonucleic Acid; dNTP = Deoxyribonucleotides; dATP = Deoxyadenosine Triphosphate; dCTP = Deoxycytidine Triphosphate; dGTP = Deoxyguanosine Triphosphate; dTTP = Deoxythymidine Triphosphate; FDA = Food and Drug Administration; FRET = Fluorescence Resonance Energy Transfer; MRSA = Methicillin Resistant Staph aureus; MTB = Mycobacterium tuberculosis; NASBA = Nucleic Acid Sequence Based Amplification; PCR = Polymerase
Chain Reaction; PFGE = Pulsed-Field Gel Electrophoresis; RT-PCR = Reverse Transcription Polymerase
Chain Reaction; Taq = Thermus aquaticus; TMA = Transcription-Mediated Amplification; VRE = Vancomycin Resistant Enterococcus.
chain reaction, or PCR, found its way into many areas in biology very soon after its invention by the American Kary Mullis in 1983, who received a Nobel Prize for his discoveries in 1993.
After a decade-long process devising a way first to initiate the process of transcription in a test tube and then to stall it by withholding one of the building blocks of RNA, they spent the next decade purifying and crystallizing the complex by exploiting a phenomenon called bilateral lipid diffusion, which allowed Kornberg and his team to create a viable, nonrestrictive crystal film to analyze the RNA polymerase
Working out the structure of RNA polymerase
was "a marvelous achievement," says James T.
Only 1 TOSV-positive pool was observed with primers specific to TOSV polymerase
and N genes, respectively.
Tokyo, Japan, June 5, 2006 - (JCN) - Takara Bio announced on June 1 that it will begin marketing two new enzymes, PrimeSTAR Max DNA Polymerase
and PrimeScript, on June 18.