polymerase chain reaction


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polymerase chain reaction

n. Abbr. PCR
A technique for amplifying DNA sequences in vitro by separating the DNA into two strands and incubating it with oligonucleotide primers and DNA polymerase. It can amplify a specific sequence of DNA as many as one billion times and is important in biotechnology, forensics, medicine, and genetic research.

pol′ymerase chain′ reac`tion



n.
the laboratory production of numerous copies of a gene by separating the two strands of the DNA containing the gene segment, marking its location with a primer, and using a DNA polymerase to assemble a copy alongside each segment and continuously copy the copies. Abbr.: PCR
Translations
amplification en chaîne par polymérase
References in periodicals archive ?
A new market study, titled "Global Polymerase Chain Reaction Consumable Market Size, Status and Forecast 2019-2025", has been featured on WiseGuyReports.
[UKPRwire, Mon May 13 2019] A new report titled " Polymerase Chain Reaction (PCR) Consumables Market Outlook and Growth Stance Forecasted Through 2026 " has been included in the enormous research repository of Market Research Reports Search Engine (MRRSE) that compiles various facets of the Polymerase Chain Reaction Consumables market at a global level portraying a holistic analysis of the marketplace along with intelligence on key participants.
[ClickPress, Mon May 13 2019] A new report titled " Polymerase Chain Reaction (PCR) Consumables Market Outlook and Growth Stance Forecasted Through 2026 " has been included in the enormous research repository of Market Research Reports Search Engine (MRRSE) that compiles various facets of the Polymerase Chain Reaction Consumables market at a global level portraying a holistic analysis of the marketplace along with intelligence on key participants.
Performance of this polymerase chain reaction method was compared against the microscopy results.
Scholtka and colleagues used 80 human colon tissue samples representing cancers and precancers to detect genetic variations using a combination of two techniques: The first technique - locked nucleic acid (LNA)-based, wild-type blocking (WTB) polymerase chain reaction - suppressed normal DNA present in large quantities in the sample; and the second technique - high-resolution melting (HRM) - enhanced the detection of genetic variations.
ABBREVIATIONS: bDNA = Branched DNA; DNA = Deoxyribonucleic Acid; dNTP = Deoxyribonucleotides; dATP = Deoxyadenosine Triphosphate; dCTP = Deoxycytidine Triphosphate; dGTP = Deoxyguanosine Triphosphate; dTTP = Deoxythymidine Triphosphate; FDA = Food and Drug Administration; FRET = Fluorescence Resonance Energy Transfer; MRSA = Methicillin Resistant Staph aureus; MTB = Mycobacterium tuberculosis; NASBA = Nucleic Acid Sequence Based Amplification; PCR = Polymerase Chain Reaction; PFGE = Pulsed-Field Gel Electrophoresis; RT-PCR = Reverse Transcription Polymerase Chain Reaction; Taq = Thermus aquaticus; TMA = Transcription-Mediated Amplification; VRE = Vancomycin Resistant Enterococcus.
The polymerase chain reaction, or PCR, found its way into many areas in biology very soon after its invention by the American Kary Mullis in 1983, who received a Nobel Prize for his discoveries in 1993.
Taking advantage Of a then-novel DNA-copying technique known as polymerase chain reaction, the colleagues searched for genes that encode olfactory receptors in rats.
Techniques mastered during the program include agarose, polyacrylamide and protein gel electrophoresis, DNA sequencing, gene mapping, restriction enzyme cleavage of DNA, recombinant gene technology, cloning, polymerase chain reaction (PCR), reverse transcription polymerase chain reaction (rtPCR), Southern and Western blotting, analysis of restriction fragment polymorhisms (RFLP), analysis of variable number tandem repeats (VNTR's), DNA fingerprinting for paternity and forensics analysis, protein analysis and bioprocessing.
Their histologic features are similar to those of papillomatous lesions in the airway, and polymerase chain reaction may identify human papillomavirus (HPV) DNA in the specimen.
Real-time polymerase chain reaction (RT-PCR) and flow cytometry were used to quantify elevated cytokine production as an indicator of inflammatory responses to four commercial dental resins.
The polymerase chain reaction assay improves the specificity and sensitivity of Trichomonas vaginalis diagnosis, compared with standard methods, Dr.

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