polymerase chain reaction

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Related to polymerase chain reactions: PCR amplification

polymerase chain reaction

n. Abbr. PCR
A technique for amplifying DNA sequences in vitro by separating the DNA into two strands and incubating it with oligonucleotide primers and DNA polymerase. It can amplify a specific sequence of DNA as many as one billion times and is important in biotechnology, forensics, medicine, and genetic research.
American Heritage® Dictionary of the English Language, Fifth Edition. Copyright © 2016 by Houghton Mifflin Harcourt Publishing Company. Published by Houghton Mifflin Harcourt Publishing Company. All rights reserved.

pol′ymerase chain′ reac`tion



n.
the laboratory production of numerous copies of a gene by separating the two strands of the DNA containing the gene segment, marking its location with a primer, and using a DNA polymerase to assemble a copy alongside each segment and continuously copy the copies. Abbr.: PCR
Random House Kernerman Webster's College Dictionary, © 2010 K Dictionaries Ltd. Copyright 2005, 1997, 1991 by Random House, Inc. All rights reserved.
Translations
amplification en chaîne par polymérase
References in periodicals archive ?
Polymerase chain reactions (PCRs) were carried out in 25 [micro]L of reaction mixtures buffered with 20 mmol/L Tris-HC1 (pH 8.4), containing 10 ng of genomic DNA, 20 pmol/L of each primer, 1.5 mM Mg[Cl.sub.2], 50 mmol/L KCl, 0.2 mmol/L of each deoxynucleoside triphosphate, and 0.2 U of Taq DNA polymerase (Amersham Biosciences Corp, Piscataway, NJ, USA).
They cover mutagenesis in various microbial backgrounds, polymerase chain reaction mutagenesis, protein evolution mutagenesis, protein structure and function mutagenesis, random mutagenesis, and mutator bacterial strain mutagenesis.
In order to examine the role of MOG in the CNS, the human MOG gene was spliced using reverse transcriptase polymerase chain reaction (RT-PCR).

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